Berek Novak's Gyn 2019. Chapter 32. Early Pregnancy Loss and Ectopic Pregnancy

 Early Pregnancy Loss and Ectopic Pregnancy

BS. Nguyễn Hồng Anh



KEY POINTS

1 Spontaneous pregnancy loss is common, occurring in up to 20% of recognized

conceptions.

2 Following an ectopic pregnancy, approximately 15% of women will have a

subsequent ectopic pregnancy.

3 Laparoscopy is the preferred approach for surgical management of ectopic

pregnancies.

4 Single-dose methotrexate appears to be the treatment of choice if medical therapy is

indicated and selected.

5 Surgical management and medical therapy have similar future reproductive

outcomes.

An abnormal gestation can be either intrauterine or extrauterine. Abnormal

intrauterine pregnancy often results in pregnancy loss early in gestation. Such

losses can be related to a number of factors such as age, previous pregnancy loss,

and maternal smoking (Table 32-1). Extrauterine or ectopic pregnancy occurs

when the fertilized ovum becomes implanted in tissue other than the

endometrium. Although 70% of ectopic gestations are located in the ampullary

segment of the fallopian tube, such pregnancies may occur in other sites (Fig. 32-

1) (1). With abnormal intrauterine and extrauterine gestation, early recognition is

key to diagnosis and management. Establishing a woman’s goals for her

pregnancy may alter management. Providers often incorrectly assume that all

spontaneous abortions or abnormal pregnancies were intended pregnancies, when

it is established that approximately 50% of pregnancies are unintended and may

be undesired (2). As with all pregnancies, management must be highly dependent

on each woman’s pregnancy goals and her perceptions of the different risks.

ABNORMAL INTRAUTERINE PREGNANCY

Spontaneous Abortion

Spontaneous abortion is a pathologic process resulting in unintentional

termination of the pregnancy prior to 20 weeks’ gestation. [1] About 8% to 20%

of known pregnancies terminate in spontaneous abortion (3,4). About 80% of

spontaneous pregnancy losses occur in the first trimester; the incidence

decreases with each gestational week (5,6). A large multicenter trial indicated

the risk of loss in a pregnancy with documented fetal cardiac activity at 10 to 13

weeks gestation had a spontaneous abortion rate of 0.96% before 24 weeks

gestation (7). In women who had one prior spontaneous abortion, the rate of

spontaneous abortion in a subsequent pregnancy ranges from 13% to 20%; in

women who had three consecutive losses, the rate is 33% to 43% (8,9). Patients

should be reassured that, in most cases, spontaneous abortion does not recur. Risk

factors for spontaneous abortion include increasing maternal age, closely spaced

pregnancies (less than 3 to 6 months apart), history of previous spontaneous

abortion, maternal diabetes, and maternal smoking during pregnancy (10–14).

Age is a dose-dependent risk factor for miscarriage; in women less than 36 years

of age, when fetal cardiac activity is confirmed by ultrasound, the risk of

spontaneous abortion is less than 4.5%. For women older than 36, the risk of

spontaneous abortion rises to 10%, and above 40 years may approach 30% (15).

Spontaneous abortion can be differentiated into various categories, based on

examination findings and ultrasound findings. The following sections review each

of these categories in detail.

Table 32-1 Potential Causes of Spontaneous Pregnancy Loss

Increased maternal age

Previous spontaneous abortion

Maternal smoking

Maternal systemic disease (diabetes mellitus, infection, thrombophilia, etc.)

Maternal alcohol consumption (moderate to high)

Increasing gravidity

Amphetamine use

Chromosomal or other embryologic abnormalities

1914Anembryonic gestation

Uterine anomalies

Intrauterine device in place

Placental anomalies

Severe maternal trauma

Extremes of maternal weight

Missed Abortion

Missed abortion is defined as a nonviable intrauterine pregnancy in the presence

of a closed cervix and minimal abdominal cramping or vaginal bleeding.

Transvaginal ultrasound evaluation is key to diagnosing a missed abortion and

guidelines are available to aid in diagnosis (Table 32-2). These criteria are

guidelines. They are more conservative than the studies and data used to develop

these guidelines with the goal of eliminating the risk of intervening in what may

have been a viable intrauterine pregnancy. These guidelines should be used in

combination with clinical judgment and the patient’s desires and wishes in

deciding next steps in management.

Missed abortion can be subdivided into anembryonic gestation and embryonic

demise. Anembryonic gestation is a pregnancy where the embryo failed to

develop. This is confirmed when the mean gestational sac diameter measured by

transvaginal ultrasound is greater than 25 mm and no embryonic pole is present.

When an embryo is present with crown–rump length greater than 7 mm and no

cardiac activity, this is classified as embryonic demise and the pregnancy is

nonviable (16)



1915FIGURE 32-1 Common sites of ectopic pregnancy. (Adapted from Seeber BE, Barnhart

KT. Suspected ectopic pregnancy. Obstet Gynecol 2006;107:399–413 and Bouyer J,

Coste J, Fernandez H, et al. Sites of ectopic pregnancy: A 10 year population-based

study of 1800 cases. Hum Reprod 2002;17:3224–3230.)

Threatened Abortion

Threatened abortion is defined as any vaginal bleeding before 20 weeks of

gestation. It occurs in at least 20% of all pregnancies (17). This is distinct

from missed abortion by ultrasound documentation of an intrauterine

embryo or fetus with cardiac activity. The bleeding is usually light and may be

associated with mild lower abdominal or cramping pain (16). The differential

diagnosis in these patients includes consideration of possible cervical polyps,

vaginitis, cervical carcinoma, gestational trophoblastic disease, ectopic

pregnancy, trauma, and foreign body. On physical examination, the abdomen

usually is not tender, and the cervix is closed. Bleeding can be seen coming from

the os and usually there is no cervical motion or adnexal tenderness.

In the vast majority of cases, threatened abortion does not result in a

pregnancy loss, but may be associated with poor outcomes later in

pregnancy. In a review of over 4,500 women presenting with first trimester

vaginal bleeding, 12% with bleeding had a miscarriage which was similar to the

rate in pregnancies with no bleeding (18). There is no effective therapy for a

threatened abortion. Bed rest and progesterone treatment, although often

advocated, have been demonstrated to be ineffective and carry their own risks

1916(19). Women with first trimester vaginal bleeding with continuing pregnancies

have nearly three times the risk of preterm birth between 28 and 31 weeks as

women without bleeding and a 50% higher likelihood of preterm birth between 32

and 36 weeks (20). First trimester bleeding may be associated with higher risk of

intrauterine growth restriction, preterm premature rupture of membranes, and

placental abruption (21).

Inevitable Abortion

With an inevitable abortion, the cervical os is open and effaced prior to 20

weeks, but no tissue has passed. Most patients have crampy lower abdominal

pain and some have cervical motion or adnexal tenderness. When the cervical os

has dilated or excessive bleeding is present, the patient should be offered medical

or surgical management. Blood type and Rh determination and a complete blood

count should be obtained if there is any concern about the amount of bleeding.

Rho(D) immune globulin (RhoGAM) should be given if the patient’s blood is

Rh negative. It is acceptable to give a dose of 50 lg until 12 completed weeks;

if this dose is not available, the standard 300 lg dose may be given (22).

Incomplete Abortion

An incomplete abortion is a partial expulsion of the pregnancy tissue. Lower

abdominal cramping is invariably present and the pain may be described as

resembling labor. On physical examination, the cervix is dilated and effaced,

bleeding is present and products of conception may be visible in the cervical

canal. Often clots are admixed with products of conception. If the bleeding is

profuse the patient should be examined promptly for tissue protruding from the

cervical os; removal of this tissue with a ring forceps may reduce the bleeding. A

vasovagal bradycardia may occur and responds to removal of the tissue. A

complete blood count, maternal blood type, and Rh determination should be

obtained; Rh-negative patients should receive RhoGAM. If the patient is

febrile, broad-spectrum antibiotic therapy should be administered.

Management of Spontaneous Abortion

All discussions of management of early pregnancy should start with asking the

woman about her pregnancy intentions and reproductive planning. Providers often

incorrectly assume that all spontaneous abortions were intended pregnancies,

when it is established that approximately 50% of pregnancies are unintended and

may be undesired (2). Establishing a woman’s goals for her pregnancy may alter

management. In women with stable vital signs and mild vaginal bleeding, three

management options exist: expectant management, medical treatment, and suction

1917curettage.

For expectant management, although there is a wide range (25% to 76%) of

success cited in the literature, it may be a desirable option for a stable and

carefully counseled patient (23,24). Success of expectant management varies

based on the type of miscarriage and patients should be counseled accordingly.

Incomplete, missed, and anembryonic abortions have a 91%, 76%, and 66%

success rates respectively (25). Women should be counseled that it may take up to

4 to 8 weeks for the pregnancy to pass in any of these situations (26). Expectant

management is associated with an increase in unscheduled surgical evacuations,

bleeding and blood transfusions but no difference in infection rates (26,27).

Medical management with misoprostol is often successful in avoiding a

surgical procedure. Misoprostol can be used vaginally, orally, sublingually, or

bucally and the dose typically ranges from 400 to 800 μg. The American College

of Obstetricians and Gynecologists recommends medical treatment of missed

abortions with 800 μg of misoprostol placed vaginally with efficacy up to 84% in

achieving complete abortion (28,29). It is acceptable to use 600 μg sublingually.

For incomplete abortion, the misoprostol dose can be reduced to 600 μg orally or

400 μg sublingually, with efficacy greater than 90% (30,31).

Studies have investigated the benefit of adding mifepristone to the medical

regimen in the hopes of improving success rates as the use of mifepristone with

misoprostol has been well-documented in the effective treatment of medical

abortion terminations. A study of 300 women with early pregnancy loss

demonstrated improved success of misoprostol when women are pretreated with

200 mg of mifepristone 24 hours prior. Approximately 83% of the women in the

group pre-treated with mifepristone experienced treatment success without

intervention by the first follow-up visit compared with only 67% in the

misoprostol only group. There were no significant differences in adverse events,

bleeding intensity, pain, or satisfaction between the two groups (32).

Suction curettage should be performed in women who desire surgical

management or who have excessive bleeding, unstable vital signs, or in whom

reliable follow-up is a concern.

ECTOPIC GESTATION

Incidence

The incidence of ectopic pregnancy has historically been underestimated.

Previous reports mainly had used hospital records and many ectopic pregnancies

are treated on an outpatient basis either in surgery centers or in outpatient clinics

with methotrexate (33). Reports using insurance claims databases have estimated

the overall rate of ectopic pregnancy to be between 1% and 2%. In one study

1918using the insurance claims database of private insurance the rate of ectopic was

0.64%, with significant differences by age group. The rate of ectopic pregnancy

increases with age, with 0.3% of pregnancies among 15- to 19-year-old women

and 1% of pregnancies among 24- to 44-year-old women (34). This study found

that up to 16% of women who present with first trimester bleeding, pain, or both,

will ultimately be diagnosed with ectopic pregnancy (35). While uncommon,

ectopic pregnancy accounts for approximately 2.7% of all maternal deaths and

increases the risk of recurrence in future pregnancies (35). Studies have shown a

decreasing mortality rate in ectopic pregnancy, attributed to improved early

diagnosis and treatment. Similar to most causes of maternal mortality, the

mortality from ectopic pregnancy varies by race, with African American women

having 6.8 times the risk of mortality from ectopic pregnancy than Caucasian

American women (36).

Etiology and Risk Factors

Ectopic pregnancy results from various factors that interrupt the successful

migration of the conceptus to the endometrium. Up to half of women who present

with an ectopic pregnancy have no identifiable risk factor, therefore a high index

of suspicion is critical. Any condition that delays or interferes with the passage of

an embryo through the fallopian tube increases the risk of ectopic pregnancy. [2]

The most important risk factor for an ectopic pregnancy is a prior ectopic

pregnancy with a recurrent risk of 10% to 15% after first ectopic pregnancy

and 30% after second ectopic pregnancy (37). Other risk factors include a

history of tubal surgery including tubal ligation, assisted reproductive technology,

and history of pelvic inflammatory disease (PID). Anything that causes

inflammation of the fallopian tube or disruption of tubal motility increases the

risk of ectopic pregnancy. Inflammation of fallopian tube is present in up to 90%

of ectopic pregnancies with causes including PID, tubal endometriosis, and

salpingitis isthmica nodosa. Many other risk factors, including smoking and

multiple lifetime sexual partners, are weakly associated with ectopic pregnancy.

Intrauterine devices (IUDs) are an effective contraceptive method, with an annual

failure rate of <0.1%. While the overall risk of pregnancy is incredibly low in

women using this type of contraception, those rare pregnancies that do occur are

more likely to be ectopic (38).

Prior Ectopic Pregnancy

A previous history of ectopic pregnancy is the strongest risk factor for another

occurrence. The increased recurrence exists because of the previous factors that

led to the initial ectopic combined with the potential damage to the fallopian tube

1919from the prior ectopic pregnancy and its treatment. The rates for intrauterine

pregnancy (40%) and ectopic pregnancy (15%, range 4% to 28%) are

similar after tubal removal or conservation (39). Tubal patency appears to be

variably affected by medical treatment with methotrexate. In one study of 121

women who received methotrexate treatment, hysterosalpingograms (HSGs) done

3 months after treatment revealed 72% of women had bilateral tubal patency, 19%

had unilateral tubal patency, and 3% had bilateral tubal obstruction. Of the

women who had bilateral tubal patency on HSG, over 90% had a subsequent

pregnancy, 78% without reproductive assistance. Given that 97% of women in the

study had no change in their fertility, an HSG is not indicated or cost-effective

after methotrexate treatment (40).

Tubal Surgery

It is clear that tubal surgery is associated with an increased risk for ectopic

pregnancy, what is unclear is whether the increased risk results from the

surgical procedure or from the underlying problem. Although tubal

sterilization remains one of the most effective forms of contraception and

pregnancy is unlikely, failures do occur; when they do, they are more likely to

result in ectopic gestation. A large Cochrane review published in 2016 found that

depending on the technique, the failure rate of tubal sterilization ranges from

36.5/1,000 procedures (Filshie clip) to 7.5/1,000 procedures (postpartum

salpingectomy and unipolar coagulation) (41). The 10-year cumulative

incidence of tubal pregnancy after any sterilization procedure is 7.3 per

1,000 procedures (42). The risk depends on the sterilization technique and the

woman’s age at the time of sterilization: postpartum partial salpingectomy and

unipolar coagulation have the lowest rates of ectopic pregnancy (1.5 and 1.8 per

1,000 procedures), while bipolar coagulation techniques had the highest incidence

(17.1 per 1,000 procedures). Spring clip and band application techniques have 10-

year ectopic rates similar to the general incidence, 8.5 and 7.3 per 1,000

procedures, respectively (42). Women younger than 28 years at the time of

sterilization are more likely to have a failure than women over 34 years.

Sterilization reversal increases risk for ectopic pregnancy. The exact risk

depends on the method of sterilization, site of tubal occlusion, residual tube

length, coexisting disease, and surgical technique. In general, the risk of any

pregnancy after a reanastomosis of a cauterized tube is up to 8% for laparoscopic

procedures based on recent studies (43). There is insufficient evidence for a rate

of ectopic pregnancy after hysteroscopic sterilization, but retrospective data and

models show overall pregnancy rates are higher than with laparoscopic

sterilization (44,45).

1920Pelvic Infection

The relationship of pelvic infection, tubal obstruction and ectopic pregnancy is

well-documented. In a study of 2,500 women with suspected PID who underwent

diagnostic laparoscopy, the incidence of ectopic pregnancy in the subsequent

pregnancy for those with laparoscopically confirmed disease was 9.1% compared

with 1.4% in the women with normal laparoscopy (46). In a study of 415 women

with laparoscopically proven PID, the incidence of tubal obstruction increased

with successive episodes of PID: 13% after one episode, 35% after two, and

75% after three (47). Chlamydia is an important pathogen causing tubal

damage and subsequent tubal pregnancy. Chlamydia was cultured from 7%

to 30% of patients with tubal pregnancy (48). Conception is three times as

likely to be tubal in women with anti–Chlamydia trachomatis titers higher than

1:64 than in those women whose titers were negative (49). Women at risk for

chlamydia infections should be diligently tested, treated when infection is present,

and counseled about the risk of ectopic pregnancy.

Infertility

The incidence of ectopic pregnancy increases with age and parity, and there is a

significant increase in nulliparous women undergoing infertility treatment

(38,39,50). Additional risks for infertile women are associated with specific

treatments, including reversal of sterilization, ovulation induction, and in vitro

fertilization (IVF). Various studies examining risk factors for ectopic pregnancy

found that infertility increased the odds of tubal pregnancy at least four times and

perhaps as much as 40 times, depending on etiology of infertility (51).

Smoking

Alterations of tubal motility, ciliary activity, and blastocyst implantation are

associated with nicotine intake. Cigarette smoking is associated with an increased

risk for tubal pregnancy in a dose-dependent fashion. Two classic case-control

studies demonstrated this relationship; compared with nonsmokers, smokers of

more than 20 cigarettes a day had a relative risk of 3.5, and smokers of up to 10

cigarettes a day had a relative risk of 2.3 (1,52).

Contraceptive Use

By reducing the overall likelihood of pregnancy, all contraceptive use reduces the

overall risk of ectopic pregnancy. There is concern that because of the various

mechanisms of action of contraceptives, if a pregnancy were to occur, it might be

more likely to be ectopic. In a meta-analysis of 13 studies examining the

relationship between contraception and the risk of ectopic pregnancy, there was

no increased risk in users of oral contraceptives or barrier methods compared with

1921pregnant controls (53). There is no demonstrated increased risk in users of depomedroxyprogesterone injections, emergency contraceptive pills or etonogestrel

implants (54,55). Hormonal and copper-containing IUDs are highly effective at

preventing intrauterine and extrauterine pregnancies, with failure rates of 0.2% in

the first year of use and 0.7% in the first 5 years of use (56). In the rare case that

women conceive with an IUD in place the pregnancy is more likely to be

ectopic.

Diagnosis

The diagnosis of ectopic pregnancy is complicated by the wide spectrum of

clinical presentations from asymptomatic cases to acute abdomen and

hemodynamic shock. Until the location of the pregnancy is confirmed, the

diagnosis remains a pregnancy of unknown location. The differential diagnosis of

a pregnancy of unknown location includes early viable intrauterine pregnancy,

early nonviable intrauterine pregnancy, stable ectopic pregnancy and unstable

ectopic pregnancy. Making the diagnosis involves combining history and physical

examination with clinical findings including laboratory and ultrasound data.

While the management of a ruptured ectopic pregnancy is directed at the

primary goal of achieving hemostasis, the management of a pregnancy of

unknown location in a stable patient is varied and depends on many patient and

pregnancy-specific factors. It is critical to maintain a high degree of suspicion of

ectopic pregnancy with pregnancies of unknown location, especially in areas of

high prevalence. History and physical examination identify patients at risk,

improving the probability of detection of ectopic pregnancy before rupture occurs.

History

The patient’s history can be helpful in identifying patients at risk for ectopic

pregnancies. Pertinent points in the history include the patient’s age, menstrual

history, previous pregnancy, infertility history, current contraceptive status, risk

factor assessment, and current symptoms.

The classic symptom triad of ectopic pregnancy is pain, amenorrhea, and

vaginal bleeding. This symptom group is present in about 50% of patients

and is more typical in patients with a ruptured ectopic pregnancy. Abdominal

pain is the most common presenting symptom, but the severity and nature of the

pain vary widely. There is no pathognomonic pain for an ectopic pregnancy. Pain

may be unilateral or bilateral and may occur in the upper or lower abdomen. The

pain may be dull, sharp, or crampy and either continuous or intermittent. With

rupture, the patient may experience transient relief of the pain, as stretching of the

tubal serosa ceases. Shoulder and back pain, thought to result from peritoneal

1922irritation of the diaphragm may indicate intra-abdominal hemorrhage.

Physical Examination

The physical examination should include measurements of vital signs and

examination of the abdomen and pelvis. Frequently, the findings before rupture

and hemorrhage are nonspecific and vital signs are normal. The abdomen may be

nontender or mildly tender, with or without rebound. The uterus may be slightly

enlarged with findings similar to a normal pregnancy (57). Cervical motion

tenderness may or may not be present. An adnexal mass may be palpable in up

to 50% of cases, but the mass varies markedly in size, consistency, and

tenderness. A palpable mass may be the corpus luteum and not the ectopic

pregnancy. With rupture and intra-abdominal hemorrhage, the patient develops

tachycardia followed by hypotension. Bowel sounds are decreased or absent. The

abdomen is distended with marked tenderness and rebound tenderness. Cervical

motion tenderness is present. Frequently, the findings of the pelvic examination

are inadequate as a result of pain and guarding. History and physical examination

may or may not provide useful diagnostic information. Additional tests are

frequently required to differentiate between early viable intrauterine pregnancy,

abnormal intrauterine pregnancy, and suspected ectopic.

Laboratory Assessment

Quantitative β-human chorionic gonadotropin (β-hCG) measurements are the

diagnostic cornerstone for ectopic pregnancy. Urine pregnancy tests can detect β-

hCG at ≥20 mIU/mL while serum pregnancy tests can detect levels >5 mIU/mL.

a-hCG levels cannot be used to predict gestational age (58). a-hCG levels

peak at approximately 10 weeks gestation and the average peak level is

approximately 100,000 with a wide normal range of variation. There is the

possibility of a phantom β-hCG, in which the presence of heterophile antibodies

or proteolytic enzymes causes a low level false-positive serum β-hCG result.

These antibodies are large glycoproteins and are not excreted in the urine,

resulting in a negative urine pregnancy test. In the patient with a-hCG levels

less than 1,000 mIU/mL, a urine pregnancy test should be performed and

confirmatory positive results obtained before instituting treatment (59).

Single Human Chorionic Gonadotropin Level

A single a-hCG measurement has limited usefulness in the evaluation of a

pregnancy of unknown location because there is considerable overlap in

values between normal and abnormal pregnancies at a given gestational age.

a-hCG levels do not correlate with pregnancy site (60). Many patients with

pregnancy of unknown location are uncertain about their menstrual dates. A

1923single β-hCG level may be useful if negative to exclude the diagnosis of ectopic

pregnancy. A single β-hCG level may facilitate the interpretation of

ultrasonography when an intrauterine gestation is not visualized. An a-hCG level

greater than the ultrasound discriminatory zone indicates a possible

extrauterine pregnancy; however caution must be used with previously

defined discrimination zones, as recent research indicates that a much higher

a-hCG level should be used for ruling out a normal intrauterine pregnancy

(16,61). Determination of serial β-hCG levels are usually needed to differentiate

an ectopic pregnancy from an intrauterine pregnancy failure. Further tests are

required for patients in whom ultrasonography examination results are

inconclusive and β-hCG levels are below the discriminatory zone.

Serial Human Chorionic Gonadotropin Level

Serial a-hCG levels are usually required when the results of the initial

ultrasonography examination are indeterminate (i.e., when there is no

evidence of an intrauterine gestation or extrauterine findings consistent with

an ectopic pregnancy). Traditionally the β-hCG level was expected to rise at

least 66% over 48 hours with an 85% confidence interval, with 15% of normal

pregnancies falling outside of this range. About the same number of ectopic

pregnancies will have a greater than 66% rise (62). Data indicate that a more

conservative cut-off of 53% gives a 99% confidence interval with less than 1% of

viable intrauterine pregnancies having a slower rise (63). This data was based on

a homogeneous patient population and women with unknown dating (i.e., unclear

last menstrual period) were excluded from the study. A more conservative cut-off

of greater than or equal to 35% was found in a larger study of over 1,000

ethnically and racially diverse women to adequately predict a viable intrauterine

pregnancy and decrease the chance of misclassifying a pregnancy (64). This large

study showed that having at least three serial values is helpful, especially if the

starting β-hCG level is low. Follow-up research suggests that the expected rise in

β-hCG is dependent upon starting β-hCG level. If the initial a-hCG level is less

than 1,500, 1,500 to 3,000, or greater than 3,000 mIU/mL, then the predicted

rise at 48 hours is 49%, 40%, and 33%, respectively (65). Based on these data

the traditional 66% should no longer be used in diagnosing ectopic pregnancies.

A cut-off of 53% or even lower to 35% may be more appropriate and

decrease the risk of intervening in what may be a viable intrauterine

pregnancy. β-hCG levels alone usually are not enough to make a diagnosis in a

pregnancy of unknown location. The entire clinical picture including history,

physical examination, and ultrasound findings, combined with β-hCG levels, are

needed to make an accurate diagnosis.

If there is a plateau or decline in β-hCG levels, this is usually indicative of a

1924nonviable pregnancy, either intrauterine or extrauterine. The decline in β-hCG

levels can be helpful in diagnosing a pregnancy of unknown location. Ectopic

pregnancies will plateau or have a slower rate of decline than spontaneous

abortions. Several studies have evaluated the expected decline to develop clinical

guidelines. Data support that most spontaneous abortions are expected to decrease

21% to 35% 2 days after presentation and 60% to 84% at 7 days. The drop

depends on the starting β-hCG level with faster decline noted with higher starting

values of β-hCG (63). An updated model suggested that the range of the drop

should be 35% to 50% at 2 days and 66% to 87% at 7 days from the first

laboratory test (66). A large study did show that if there is an 85% drop within 4

days or a 95% drop in 7 days the risk of ectopic pregnancy appears to be 0 (67).

These expected rates of decline can aid in detecting those women at risk for an

ectopic pregnancy and warrant closer follow-up or even intervention, but should

be combined with clinical judgment and not be used as absolutes in patient

management.

Serum Progesterone

Earlier studies suggested that serum progesterone level could be used to diagnose

ectopic pregnancies and identify viable intrauterine pregnancies. However, the

level of progesterone in all pregnancies can vary greatly. There are progesterone

thresholds that can aid in the evaluation of patient with pregnancy of unknown

location. Progesterone levels greater than or equal to 25 ng/mL are associated

with viable intrauterine pregnancies as only 1% to 2% of ectopic pregnancies will

have levels this high (68). If the ectopic pregnancy does have a level above this

threshold it is usually associated with cardiac activity and identifiable on

ultrasound. Serum progesterone levels <5 mg/mL are associated with pregnancy

failure. Less than 1% of viable intrauterine pregnancies are below this cut-off

(69,70).

Other Endocrine Markers

In an effort to improve early detection of ectopic pregnancy various endocrine

and protein markers have been evaluated. Useful biomarkers have not been

identified for clinical use but further studies are underway.

Ultrasonography

Transvaginal ultrasound is the imaging modality of choice for evaluating the

pelvic structures and the location of a newly diagnosed pregnancy (71,72).

Transabdominal ultrasonography permits visualization of the pelvis and

abdominal cavity and should be included as part of the complete ectopic

pregnancy evaluation to detect adnexal masses and hemoperitoneum.

1925The earliest ultrasound finding of an intrauterine pregnancy is the

gestational sac that characteristically has a round, thick echogenic ring

surrounding a sonolucent center. As the pregnancy progresses this sac becomes

eccentrically located within the endometrial cavity. Although the gestational sac

is the first sign of a definitive intrauterine pregnancy it can be mimicked by an

intrauterine fluid collection called a pseudogestational sac. Pseudosacs occur in

8% to 29% of patients with ectopic pregnancy (73,74). This ultrasonographic

lucency, centrally located, probably represents bleeding into the endometrial

cavity by the decidual cast. Clots within this lucency may mimic a fetal pole.

Historically, identification of the double decidual sac sign (DDSS) is the best

method of differentiating true sacs from pseudosacs. The double sac, believed to

be the decidua capsularis and parietalis, is seen as two concentric echogenic rings

separated by a hypoechogenic space. Although useful, this approach has some

limitations in sensitivity and specificity—the DDSS sensitivity ranges from 64%

to 95% (75). Pseudosacs may occasionally appear as the DDSS; intrauterine sacs

of failed pregnancies may appear as pseudosacs. The DDSS is best observed on

transabdominal ultrasound as this is frequently missed with transvaginal

ultrasound (16). However, if abdominal ultrasound reveals a DDSS with an

empty-appearing gestational sac, a transvaginal ultrasound should be performed

as a yolk sac is often visible in this view and is a definitive diagnosis of

intrauterine pregnancy.

The appearance of a yolk sac within the gestational sac is diagnostic of an

intrauterine pregnancy, while the gestational sac alone is not sufficient

because of the risk of misdiagnosis (76). Diagnosing an ectopic pregnancy on

transvaginal ultrasound may or may not be straightforward. In the absence of an

intrauterine pregnancy, if an adnexal gestational sac with a yolk sac or an embryo

is present this is diagnostic of an ectopic pregnancy. However, this finding is the

least sensitive sign of ectopic pregnancy, occurring in only 10% to 17% of cases

(77).

The recognition of other characteristics of ectopic pregnancy improves

ultrasonographic sensitivity. Adnexal rings (fluid sacs with thick echogenic rings)

are visualized in less than 50% of ectopic pregnancies (77,78). The adnexal ring

may not always be apparent because bleeding around the sac results in the

appearance of a nonspecific adnexal mass. Complex or solid adnexal masses are

frequently associated with ectopic pregnancy; however, the mass may

represent a corpus luteum, endometrioma, hydrosalpinx, ovarian neoplasm

(e.g., dermoid cyst), or pedunculated fibroid (5,78). The presence of free culde-sac fluid is frequently associated with ectopic pregnancy but is not considered

evidence of rupture (79). The presence of intra-abdominal free fluid should raise

concern about tubal rupture.

1926Historically ultrasound findings were correlated with a “discriminatory

zone”—the level of β-hCG where an intrauterine pregnancy should be visualized.

Discriminatory zones for transvaginal ultrasonography are reported at levels

from 1,000 to 2,000 mIU/mL and vary by institution (15,73,75,77,78,80).

Studies have suggested raising the discriminatory zone to 3,510 mIU/mL in

order to avoid false results for an abnormal pregnancy (61). Discriminatory

zones vary according to the expertise of the examiner, capability of the

equipment, and pregnancy-related characteristics such as multiples.

Although there are guidelines for a discriminatory zone for intrauterine

pregnancy, there is no such zone for ectopic pregnancy. Levels of β-hCG do

not correlate with the size of ectopic pregnancy. Regardless of how high the β-

hCG level may be, nonvisualization of the pregnancy outside the uterus does not

exclude ectopic pregnancy. An ectopic pregnancy may be present anywhere in the

abdominal cavity, making ultrasonographic visualization difficult.

Dilation and Curettage

Uterine curettage is performed when the pregnancy is confirmed to be

nonviable or is not desired and the location of the pregnancy cannot be

determined by ultrasonography. The decision to evacuate the uterus in the

presence of a positive pregnancy test must be made with caution to avoid the

unintentional disruption of a viable intrauterine pregnancy. Although suction

curettage traditionally was performed in the operating room, it can be

accomplished under local anesthesia on an outpatient basis. Endometrial sampling

methods (e.g., a Novak curettage or Pipelle endometrial sampling device) are

accurate in diagnosing abnormal uterine bleeding but their reliability for detecting

intrauterine chorionic villi is low and these devices should not be used in the

evaluation of a possible ectopic pregnancy (81,82).

It is essential to confirm the presence of trophoblastic tissue as rapidly as

possible so that therapy may be instituted. There are many ways this tissue can

be evaluated depending on setting of procedure. Traditionally these specimens

were sent to pathology for formal evaluation. In this case, the presence of

chorionic villi may be assessed rapidly with frozen section analysis, which avoids

the waiting period of at least 48 hours for permanent histologic evaluation.

Immunocytochemical staining techniques can be used to differentiate

intermediate trophoblasts from decidual tissue (83).

More often these simple procedures are performed in an outpatient setting and

the ability to immediately evaluate the tissue allows for rapid diagnosis of an

intrauterine pregnancy. Surgeons experienced in evaluating tissue immediately

after aspiration are at least as accurate as pathologists especially with accurately

identifying the presence of the products of conception (84).

1927After tissue is obtained by curettage, it can be added to saline, in which it will

float. Decidual tissue does not float. Chorionic villi are usually identified by their

characteristic lacy frond appearance, as demonstrated in Figure 32-2 above.


FIGURE 32-2 When floated in saline, chorionic villi are often readily distinguishable as

lacy fronds of tissue. (From Stovall TG, Ling FW. Extrauterine Pregnancy: Clinical

Diagnosis and Management. New York: McGraw-Hill, 1993:186, with permission.)

It is important to identify a gestational sac. Rare villi may be present in uterine

aspirations in the presence of interstitial pregnancies, therefore identifying the

gestational sac is required. Heterotopic pregnancies are rare but are increasing

with advancements in artificial reproductive technologies. If suspicion for a

heterotopic pregnancy is high the presence of villi and a gestational sac still

1928warrants further investigation.

If chorionic villi are not confirmed β-hCG levels should be monitored. After

evacuation of an abnormal intrauterine pregnancy, the β-hCG level decreases by

greater than 15% within 12 to 24 hours (50). It has been suggested that a fall of

≥50% within 24 hours following manual vacuum aspiration is predictive of an

abnormal intrauterine pregnancy (85). A borderline fall may represent interassay

variability. A repeat level should be obtained in 24 to 48 hours to confirm the

decline. If the uterus is evacuated and the pregnancy is extrauterine, the β-hCG

level will plateau or continue to increase, indicating the presence of extrauterine

trophoblastic tissue.

Culdocentesis

Culdocentesis was historically widely used as a diagnostic technique for ectopic

pregnancy. With the use of β-hCG testing and transvaginal ultrasonography,

culdocentesis is rarely indicated and is considered obsolete in the evaluation of

ectopic pregnancies (86).

Laparoscopy

Traditionally laparoscopy was the gold standard for the diagnosis of ectopic

pregnancy. At the time of laparoscopy, the fallopian tubes are easily visualized

and evaluated, but the diagnosis of ectopic pregnancy is missed in 3% to 4% of

patients who have very small ectopic gestations. The ectopic gestation is seen

distorting the normal tubal architecture. With earlier diagnosis, the possibility

increases that a small ectopic pregnancy may not be visualized. Pelvic adhesions

or previous tubal damage may compromise assessment of the tube. False-positive

results occur when tubal dilation or discoloration is misinterpreted as an ectopic

pregnancy, in which case the tube can be incised unnecessarily and damaged.

With the advances in ultrasound technology, more ectopic pregnancies are being

diagnosed earlier allowing for medical interventions and reducing the need for

surgical management, thus laparoscopy is no longer considered the gold standard

for diagnosis.

Diagnostic Algorithm

The presenting symptoms and physical findings of patients with unruptured

ectopic pregnancies are similar to those of patients with normal intrauterine

pregnancies (87). History, risk factor assessment, and physical examination are

the initial steps in the management of suspected ectopic pregnancy. Patients in a

hemodynamically unstable condition should undergo immediate surgical

intervention. Patients with a stable, relatively asymptomatic condition may be

assessed as outpatients.

1929There are several potential benefits if the diagnosis of ectopic pregnancy can be

confirmed without laparoscopy. First, the anesthetic and surgical risks of

laparoscopy are avoided; second, medical therapy becomes a treatment option.

Because many ectopic pregnancies occur in histologically normal tubes,

resolution without surgery may spare the tube from additional trauma and

improve subsequent fertility. Previously, an algorithm for the diagnosis of

ectopic pregnancy without laparoscopy proved to be 100% accurate in a

randomized clinical trial (88,89).

This screening algorithm combined the results of history and physical

examination, serial β-hCG levels, vaginal ultrasonography, and dilation and

curettage (Fig. 32-3). Serial β-hCG levels are used to assess pregnancy viability,

correlated with transvaginal ultrasonography findings and measured serially after

a suction curettage. For patients in a stable condition, a treatment decision is

never based on a single β-hCG level. After the initial evaluation, the patient is

seen again at 48 hours for a repeat β-hCG level. At this time, transvaginal

ultrasonography often is repeated so the findings can be correlated with the two β-

hCG levels. The algorithm has been updated to account for changes in the new

recommendations for a discriminatory zone and expected changes in the β-hCG

level with a viable intrauterine pregnancy.

In this algorithm, transvaginal ultrasonography is used as follows:

1. The identification of an intrauterine gestational sac with yolk sac excludes

the presence of an extrauterine pregnancy. If the patient has a β-hCG level

of more than 3,510 mIU/mL, and no intrauterine gestational sac is identified,

the patient is considered to have an extrauterine pregnancy and can be treated

without further testing.

2. Adnexal gestational sac with a yolk sac or embryo, when seen, definitively

confirms the diagnosis of ectopic pregnancy.

3. A tubal mass as small as 1 cm can be identified and characterized.

Suction curettage is used to differentiate nonviable intrauterine

pregnancies from ectopic gestations (less than 53%, or even as low as 35%,

rise in a-hCG level over 48 hours, an a-hCG level of less than 3,510 mIU/mL,

and indeterminate ultrasonography findings). Performance of this procedure

avoids unnecessary use of methotrexate in patients with abnormal intrauterine

pregnancy that can be diagnosed only by evacuating the uterus. An unlikely

potential problem with suction curettage is missing either an early nonviable

intrauterine pregnancy or combined intrauterine and extrauterine pregnancies.

Treatment

1930Ectopic pregnancy can be effectively treated medically or surgically.

Traditionally, exploratory laparotomy with unilateral salpingectomy was used for

diagnosis and treatment for ectopic pregnancies. With techniques available that

allow for early detection, including serum quantitative β-hCG levels and

ultrasound, more conservative treatment options are available. Minimally invasive

surgical techniques and medical management with methotrexate are the

commonly used treatment options for ectopic pregnancies. The treatment

approach depends on the clinical circumstances, the site of the ectopic

pregnancy and the available resources.

Surgical Treatment

Operative management is the most widely used treatment for ectopic pregnancy.

The surgical approach (laparotomy vs. laparoscopy) and procedure

(salpingectomy vs. salpingostomy) used to treat ectopic pregnancies depend on

the clinical circumstances, available resources, and provider skill level. Each

approach and procedure has associated risks and benefits and the treatment

employed must be individualized to best meet the needs of the patient and

provider.

Laparotomy Versus Laparoscopy

Treatment of an ectopic pregnancy can be accomplished by laparoscopy or

laparotomy. The hemodynamic stability of the patient, size and location of the

ectopic mass, and the surgeon’s expertise all contribute to determining the

appropriate surgical approach. Laparotomy is indicated when the patient

becomes hemodynamically unstable and an expedited abdominal entry is

required. A ruptured ectopic pregnancy does not necessarily require

laparotomy. If the hemoperitoneum cannot be evacuated in a timely manner,

laparotomy should be considered. Surgeon’s experience with laparoscopy and the

availability of laparoscopic equipment will determine the surgical approach.

In most cases, [3] laparoscopy is superior to laparotomy for management

of ectopic pregnancy. The indications for laparotomy include abdominal

pregnancy, extensive abdominal or pelvic adhesive disease, and any other

condition making laparoscopy difficult or unsafe. In a case control study of 50

patients comparing the use of laparoscopy and laparotomy for ectopic pregnancy

management, hospital stay was significantly shorter (1.3 ± 0.8 vs. 3.0 ± 1.1 days),

operative time was shorter (78 ± 26 vs. 104 ± 27 minutes), and convalescence was

shorter (9 ± 8 vs. 26 ± 16 days) in the laparoscopy group (90).

Laparoscopic management was associated with significant cost savings

when compared with laparotomy ($4,368 ± $277 vs. $5,090 ± $168). Using a

prospective analysis, 105 patients with tubal pregnancy were stratified with regard

1931to age and risk factors and randomized to undergo either laparoscopic

management or laparotomy (91). Subsequently, 73 patients underwent secondlook laparoscopy to assess the degree of adhesion formation. Patients treated by

laparotomy had significantly more adhesions at the surgical site than those treated

by laparoscopy, but tubal patency rates were similar. A Cochrane review

confirmed these findings and laparoscopic salpingostomy was associated with

decreased cost, operative time, blood loss, and hospital stay when compared to

salpingostomy at the time of a laparotomy (92).

An alternative to laparoscopy is the use of a mini-laparotomy incision. This

approach has the advantage of not requiring laparoscopic equipment and utilizes a

smaller incision that should result in decreased postoperative pain and shorter

recovery times for patients. A randomized control trial comparing

minilaparotomy to laparotomy showed decreased complication rates and reduced

costs associated with the mini-laparotomy incision with similar success rates in

the treatment of the ectopic pregnancy (92,93).




FIGURE 32-3 Non-laparoscopic algorithm for diagnosis of ectopic pregnancy. β-hCG,

human chorionic gonadotropin; IUP, intrauterine pregnancy; D&C, dilation and curettage.

Salpingectomy Versus Salpingostomy

Multiple studies have been conducted to compare management of ectopic

pregnancy with a salpingostomy or salpingectomy. The decision to choose one

technique over the other depends on the patient’s fertility goals, clinical picture,

and the condition of the affected and contralateral fallopian tubes.

Linear salpingostomy can be considered when the patient has an unruptured

ectopic pregnancy, wishes to retain her potential for future fertility, and the

affected fallopian tube appears otherwise normal. This is particularly important to

consider if the contralateral tube appears damaged or has been previously

removed.

In a salpingostomy, the products of conception are removed through an incision

made into the tube on its antimesenteric border. The procedure can be

accomplished with needle-tip cautery, laser, scalpel, or scissors. It can be done

with operative laparoscopic techniques or via a laparotomy. Contraindications to a

salpingostomy include ruptured fallopian tube, use of extensive cautery to obtain

hemostasis, severely damaged tube and recurrent ectopic pregnancy in the same

tube. The main risk of salpingostomy is a persistent ectopic pregnancy resulting

from failure to remove the entire pregnancy. This was reported in 8% of cases in a

recent large open-label study (94). Patients with high starting β-hCG levels, early

gestations, and small ectopic pregnancies (<2 cm) are at greater risk of having a

persistent pregnancy after a salpingostomy (95). Because of this risk, after

salpingostomy, weekly β-hCG levels should be followed to ensure complete

resolution of the ectopic pregnancy. β-hCG levels that persist or plateau can

usually be treated successfully with a single dose of methotrexate (31).

Historically, milking the tube to effect a tubal abortion was advocated; if the

pregnancy is located within the fimbriae, this technique may be effective.

However, when milking was compared with linear salpingostomy for ectopic

pregnancies within the ampulla, milking was associated with a twofold increase in

the recurrent ectopic pregnancy rate, thus this process is generally not

recommended (96).

Reproductive Outcome

Reproductive outcome after ectopic pregnancy is evaluated by determining tubal

patency by HSGs, the subsequent intrauterine pregnancy rate, and the recurrent

ectopic pregnancy rate. Pregnancy rates are similar in patients treated by either

laparoscopy or laparotomy, though a minimally invasive procedure is preferred

due to lower overall risks and faster recovery. Tubal patency on the ipsilateral

1935side after conservative laparoscopic management is about 84% (97). In a

study of 143 patients followed after undergoing laparoscopic procedures for

ectopic pregnancy, the overall intrauterine pregnancy rates after

laparoscopic salpingostomy (60%) and laparoscopic salpingectomy (54%)

were not significantly different (98). However, if the patient had evidence of

tubal damage, pregnancy rates (42%) were significantly lower than in those

women who did not have tubal damage (79%). In another study, the reproductive

outcome of 188 patients followed for a mean of 7.2 years (range 3 to 15 years)

was reported after conservation by laparotomy for ectopic pregnancy (99). In

randomized controlled trials comparing salpingectomy and salpingostomy, there

was no overall difference in future intrauterine pregnancy rates or repeat ectopic

pregnancy. However, in cohort studies, salpingostomy is associated with higher

rate of repeat ectopic pregnancy (10% vs. 4% with salpingectomy, OR 2.27), but

is associated with a slightly higher rate of intrauterine pregnancy (RR 1.24, CI

1.08–1.42) (100).

Medical Treatment

The drug most frequently used for medical management of ectopic

pregnancy is methotrexate, although other agents have been studied, including

potassium chloride (KCl), hyperosmolar glucose, prostaglandins, and RU-486.

These agents may be given systemically (intravenously, intramuscularly, or

orally) or locally (laparoscopic direct injection, ultrasound-guided injection, or

retrograde salpingography). Other agents besides methotrexate are not

recommended for the treatment of ectopic pregnancy because their safety and

efficacy are not well-documented.

Methotrexate

Methotrexate is a folic acid analog that inhibits dehydrofolate reductase and

thereby prevents synthesis of DNA. Methotrexate affects actively growing cells

including trophoblastic tissues, malignant cells, bone marrow, intestinal mucosa

and respiratory epithelium (101). It is used extensively in the treatment of

gestational trophoblastic disease (see Chapter 41). Initially, methotrexate was

used for the treatment of trophoblastic tissue left in situ after exploration for an

abdominal pregnancy (102). In 1982, Tanaka et al. treated an unruptured

interstitial gestation with a 15-day course of intramuscular methotrexate and

began treatment of ectopic pregnancies with this medication (103). Multiple

studies have documented the safety and efficacy of methotrexate therapy for the

management of ectopic pregnancies; this is the first-line treatment for many

providers. Approximately 35% of patients with ectopic pregnancies are

candidates for primary therapy with methotrexate (104). Methotrexate may be

1936given for the treatment of persistent ectopic pregnancies that fail surgical

management.

Candidates for Methotrexate

Medical management of ectopic pregnancies with methotrexate is safe and

effective, however, not all patients are candidates for this medical therapy.

Methotrexate therapy can be considered for patients with confirmed, or high

suspicion for ectopic pregnancy who are hemodynamically stable with no

evidence of rupture. Table 32-3 documents the absolute contraindications to

methotrexate therapy including breastfeeding, hepatic, renal, or hematologic

disorders and known sensitivity to methotrexate. Relative contraindications to

methotrexate therapy include gestational sac greater than 4 cm and embryonic

cardiac motion on transvaginal ultrasound (Table 32-3). Prior to the

administration of methotrexate, a patient should have a complete blood count,

blood type, liver function tests, electrolyte panel including creatinine, and a chest

x-ray if there is any history of pulmonary disease. These studies are usually

repeated 1 week after administration of methotrexate to evaluate for any potential

complications from the therapy (105).

Table 32-3 Contraindications to Medical Therapy

Absolute Contraindications

Intrauterine pregnancy

Hemodynamically unstable

Ruptured ectopic pregnancy

Breastfeeding

Immunodeficiency

Moderate to severe anemia, leukopenia or thrombocytopenia

Known sensitivity to methotrexate

Active pulmonary disease

Active peptic ulcer disease

Clinically important hepatic or renal dysfunction

Relative Contraindications

1937Ectopic pregnancy >4 cm in size by transvaginal ultrasound

Embryonic cardiac motion detected on transvaginal ultrasound

Unable to comply with medical management follow-up

High initial β-hCG concentration (>5,000 mIU/mL)

Refusal to accept blood transfusion

Adapted from The Practice Committee of the ASRM, Medical Treatment of Ectopic

Pregnancy, 2013.

Methotrexate Dosing Regimens

Methotrexate is typically given via intramuscular injection but can be

administered orally or by intravenous infusion. There are three published

regimens for administration of methotrexate: single-dose regimen, a two-dose

regimen, and a multidose regimen. Methotrexate traditionally was administered

using a multidose regimen, but single dosing protocols have improved patient

compliance and have similar success rates of approximately 90% (92,106). The

single-dose protocol also has fewer side effects, however an additional dose of

methotrexate may be required in up to 25% of women who receive this treatment

(92,106). This second dose is more often required in women with higher starting

β-hCG levels.

Methotrexate Single-Dose Regimens

Single-dose regimens were designed to increase patient compliance and simplify

the administration of methotrexate. This regimen is well studied and safe and

effective in the treatment of ectopic pregnancies. The single-dose regimen is

detailed in Table 32-4.

Table 32-4 Methotrexate Treatment Regimens

Single-Dose Regimen

Administer MTX 50 mg/m2 on day 0

Measure β-hCG level on days 4 and 7

If levels drop by 15%, monitor β-hCG weekly until nonpregnant level

If levels do not drop by 15%, repeat dose of MTX and measure β-hCG on days 4

and 7

1938Multidose Regimen

Administer MTX 1 mg/kg IM days 1, 3, 5, 7

Administer leucovorin 0.1 mg/kg IM days 2, 4, 6, 8

Measure β-hCG levels on days 1, 3, 5, 7 until 15% decrease between two

measurements

Once β-hCG levels drop 15%, stop MTX and monitor β-hCG weekly until

nonpregnant level

Two-Dose Regimen

Administer MTX 50 mg/m2 on days 0 and 4

Measure β-hCG level on days 4 and 7

If levels drop by 15%, monitor β-hCG weekly until nonpregnant level

If levels do not drop by 15%, repeat dose of MTX on days 7 and 11 and measure

β-hCG on days 11 and 14. If levels drop 15%, monitor β-hCG weekly until

nonpregnant level

MTX, methotrexate; IM, intramuscular; β-hCG, β-human chorionic gonadotropin.

Adapted from The Practice Committee of the ASRM, Medical Treatment of Ectopic

Pregnancy, 2013.

Approximately 15% to 20% of patients in the single-dose regimen will require

a second dose of methotrexate because of persistent β-hCG levels (106,107). The

β-hCG level at the time of treatment appears to predict the subsequent success

rate of single-dose therapy. Patients with β-hCG levels greater than 5,000

mIU/mL have a 14.3% chance of treatment failure compared to only 3.7% for

women with levels less than 5,000 mIU/mL. [4] Compared with the multidose

protocol, single-dose methotrexate is less expensive, patient acceptance is

greater because less monitoring is required during treatment, and the

treatment results and prospects for future fertility are comparable (108).

Multidose Regimen

The multidose regimen is outlined in Table 32-4. Patients receive 1 mg/kg of

methotrexate intramuscularly or intravenously on days 1, 3, 5, and 7 with

leucovorin 0.1 mg/kg administered on days 2, 4, 6, and 8. As a result of the repeat

dosing of methotrexate, side effects are more common. The leucovorin helps

reduce these side effects and increases patients’ tolerance of the treatment. A

1939patient may not require all four doses of methotrexate and her β-hCG levels

should be monitored on days 1, 3, 5, and 7. If the β-hCG level drops 15% between

two measurements, the regimen can be stopped and weekly β-hCG monitoring

initiated. If the methotrexate is discontinued early, the patient should receive

leucovorin after her final dose of methotrexate to help reduce potential side

effects. If a patient’s β-hCG level plateaus or increases, a second round of

methotrexate and leucovorin can be given 1 week later. Earlier studies indicated

approximately 19% will require all four doses, and 17% of women will require

only one dose with this regimen (109,110). A meta-analysis showed 10% of

women require only one dose, while nearly 54% will require all four doses

(106).

Methotrexate Two-Dose Regimen

The two-dose regimen was described as a cross between the single- and multidose

regimens. Because only 54% require all four doses of the multidose regimen and

15% to 25% will require a second dose in the single-dose regimen, it is

reasonable to consider a two-dose regimen of methotrexate. The protocol is

outlined in Table 32-4 and involves the administration of methotrexate on day 0

and day 4 with monitoring of β-hCG levels on days 4 and 7. If there is less than a

15% drop between the two measures, a repeat administration of methotrexate is

given on days 7 and 11 and β-hCG levels drawn accordingly. A single study

showed an 87% success rate with low complication rates and high patient

satisfaction (111).

Effectiveness of Methotrexate

The overall effectiveness of methotrexate therapy ranges from 78% to 96% (112).

Although the previously mentioned meta-analysis of 26 observational studies

including 1,300 women revealed a slightly improved rate of success with

multidose therapy, another meta-analysis including two randomized controlled

trials showed no difference in success rates between the two regimens. However,

the β-hCG levels in both of those studies were less than 3,000 mIU/mL

(106,113,114). Systematic reviews have demonstrated an overall failure rate of at

least 14.3% when initial β-hCG is greater than 5,000 mIU/mL compared with a

failure rate of less than 4% when the β-hCG is less than 5,000 mIU/mL (108).

When comparing methotrexate to laparoscopic salpingostomy, the multidose

regimen has similar success rates. The single-dose regimen has lower initial

success rates. However, after women receive additional doses as needed, the

success rates are comparable between laparoscopic salpingostomy and singledose methotrexate protocols (106).

Table 32-5 Initiation of Methotrexate: Physician Checklist and Patient Instructions

1940Physician Checklist

Obtain β-hCG level

Check CBC with differential, liver function tests, creatinine, blood type, and

antibody screen

Administer RhoGAM if patient is Rh-negative

Identify unruptured ectopic pregnancy smaller than 4 cm (relative contraindication)

Obtain informed consent

Prescribe FeSO4 325 mg PO bid if hematocrit is less than 30%

Schedule follow-up appointment on days 4 and 7

Patient Instructions

Refrain from alcohol use, multivitamins containing folic acid, NSAID use and sexual

intercourse until β-hCG level is negative

Call your physician if:

You experience prolonged or heavy vaginal bleeding

The pain is prolonged or severe (lower abdomen and pelvic pain is normal during

the first 10–14 days of treatment)

About 4% to 5% of women experience unsuccessful methotrexate treatment and require

surgery. β-hCG, human chorionic gonadotropin; SGOT, serum glutamic-oxaloacetic

transaminase; BUN, blood urea nitrogen; CBC, complete blood count; RhoGAM, Rho(D)

immune globulin; NSAIDs, nonsteroidal anti-inflammatory drugs; WBC, white blood cell;

PO, by mouth; bid, twice daily.

Initiating Methotrexate

Outlined in Table 32-5 is a checklist that should be followed by the physician

before initiating methotrexate. It includes instructions that are helpful to the

patient.

Patient Follow-Up

After intramuscular administration of methotrexate, regardless of the dose

regimen used, patients are monitored on an outpatient basis with weekly β-

hCG levels. These levels need to be monitored until the β-hCG reaches

nonpregnant levels. It is possible that tubal rupture may occur even if β-hCG

levels are falling. Signs of a tubal rupture include severe pain, hemodynamic

1941instability, and a drop in hematocrit. Patients who report severe or prolonged pain

should be evaluated by measuring hematocrit levels and performing transvaginal

ultrasonography. The ultrasound findings during follow-up, although usually not

helpful, can be used to provide reassurance that the tube is not ruptured (115).

Cul-de-sac fluid is a common finding and the amount of fluid may increase if a

tubal abortion occurs. It is not necessary to intervene surgically, unless the patient

has a precipitous drop in hematocrit levels or she becomes hemodynamically

unstable.

Side Effects

Side effects of methotrexate therapy are dose and frequency dependent. The

most commonly reported side effects are the gastrointestinal symptoms of

nausea, vomiting, stomatitis, and abdominal pain. The frequency of reported

side effects ranges from 30% to 40% (106). As a result of these potential effects,

women are cautioned against using alcohol and nonsteroidal anti-inflammatory

medications during treatment with methotrexate. Other side effects include bone

marrow suppression, hemorrhagic enteritis, alopecia, dermatitis, elevated liver

enzyme levels, and pneumonitis (116). These side effects are usually mild and

self-limited; few life-threatening side effects are reported. The risk of all side

effects does appear to be higher for multidose regimens. For those patients on

prolonged therapy, leucovorin can reduce the incidence of these side effects and is

included in the “multidose” regimen. Long-term follow-up of women treated with

methotrexate for gestational trophoblastic disease shows no increase in congenital

malformations, spontaneous abortions, or tumors recurring after chemotherapy

(117). Treatment of ectopic pregnancy differs from that of gestational

trophoblastic disease in that a smaller total dose of methotrexate is required and

shorter treatment duration is used. Although surgical management of ectopic

pregnancy remains the mainstay of treatment worldwide, methotrexate treatment

is appropriate in those patients who meet the treatment criteria.

Reproductive Outcome

Reproductive function after methotrexate treatment can be assessed on the basis

of repeat ectopic pregnancy rates, tubal patency, and pregnancy outcome. The risk

of subsequent ectopic pregnancy is approximately 10% following either

methotrexate or salpingostomy (118,119). The tubal patency rates are reported to

be higher than 80% in those patients treated with either single-dose or multidose

regimens with no difference in rates compared with women treated with

salpingostomy. A randomized trial comparing methotrexate to laparoscopic

salpingostomy showed no difference in tubal patency rates among the two groups,

although in this trial patency rates were lower than previously reported at 66% in

1942the salpingostomy group (120). Subsequent spontaneous intrauterine pregnancy

rates are similar between those women treated with methotrexate versus

salpingostomy, with rates ranging from 36% to 64% (121,122). A Cochrane

review comparing interventions for ectopic pregnancy showed no difference in

subsequent risk of a repeat ectopic pregnancy and future intrauterine pregnancy

rates comparing surgically and medically treated patients. Studies evaluating

ovarian reserve after treatment with methotrexate for an ectopic showed no

impact on future ovarian reserve (123,124). [5] Comparison of laparoscopically

treated patients with methotrexate-treated patients indicates that the two

methods have similar reproductive outcomes (92).

Types of Ectopic Pregnancy

Spontaneous Resolution of Pregnancies of Unknown Location

Some ectopic pregnancies resolve by resorption or by tubal abortion,

obviating the need for medical or surgical therapy. The proportion of ectopic

pregnancies that resolve spontaneously and the factors that make this more likely

are unknown. There are no specific criteria for predicting successful spontaneous

resolution of an ectopic pregnancy. A falling β-hCG level is the most common

indicator used, but tubal rupture can occur even with falling β-hCG levels. One

recent study found that a ratio of β-hCG level at 48 hours compared with initial

hCG level of <0.87 predicted a failing pregnancy of unknown location, better than

absolute serum β-hCG levels at both of those time periods (125). Patients with

low initial levels of β-hCG are generally the best candidates for expectant

management, and there is a reported 88% success rate of spontaneous remission

with an initial β-hCG level less than 200 mIU/mL (126,127). These patients

should be followed with serial β-hCG levels, with a plan to initiate active

management if levels plateau or rise or signs suggestive of tubal rupture occur.

Persistent Trophoblastic Tissue

Persistent ectopic pregnancy occurs when a patient underwent conservative

surgery (e.g., salpingostomy, fimbrial expression) and viable trophoblastic

tissue remains. Histologically, several criteria exist: there is no identifiable

embryo, the implantation usually is medial to the previous tubal incision and

residual chorionic villi are confined to the tubal muscularis. Peritoneal

trophoblastic tissue implants may be responsible for persistence. Diagnosis is

made when β-hCG levels plateau after conservative surgery. Risk factors for

persistent ectopic pregnancy include the type of surgical procedure, the initial β-

hCG level, the duration of amenorrhea, and the size of the ectopic pregnancy,

with smaller, earlier pregnancies treated by salpingostomy carrying the highest

1943risk (128). Patients treated with laparoscopic salpingostomy have a higher rate of

persistent ectopic pregnancies compared to those treated with salpingostomy at

the time of a laparotomy, with an incidence of persistence after laparoscopic

linear salpingostomy ranging from 4% to 15% (39,92,94).

Management. Persistent ectopic pregnancy can be treated surgically or

medically; surgical therapy consists of either repeat salpingostomy or, more

commonly, salpingectomy. Methotrexate offers an alternative to patients who are

hemodynamically stable at the time of diagnosis. Methotrexate may be the

treatment of choice because the persistent trophoblastic tissue may not be

confined to the tube and, therefore, not readily identifiable during repeat surgical

exploration (128).

Nontubal Ectopic Pregnancy

The majority of ectopic pregnancies diagnosed are found in the ampulla portion

of the fallopian tube and the majority (93%) found within some part of the

fallopian tube. Increasingly, more ectopic pregnancies are found in other locations

within the abdomen and pelvis. These nontubal ectopic pregnancies are rare but

are important to consider as they are associated with more adverse events caused

by difficulty and delay in diagnosis and treatment.

Cervical Pregnancy

Diagnosis. Pregnancy implanted within the cervix is the rarest of all ectopic

pregnancies, accounting for less than 1% of all ectopic pregnancies (1). The

cause of cervical ectopic pregnancies is unknown and the rare occurrence

prevents identification of known risk factors. The diagnosis may not be suspected

until the patient is undergoing suction curettage for a presumed incomplete

abortion and hemorrhage occurs. In some cases, bleeding is light, whereas in

others there is hemorrhage. These types of ectopic pregnancies are diagnosed and

distinguished from ongoing spontaneous abortion mainly with ultrasound

findings, and serial β-hCG measurements. Distinguishing a cervical ectopic

pregnancy from an incomplete process is often difficult, and many cervical

ectopic pregnancies may be misdiagnosed (129). Suggested criteria can be found

in Table 32-6. Other potential diagnoses that must be differentiated from cervical

pregnancy include cervical carcinoma, cervical or prolapsed submucosal

leiomyomas, trophoblastic tumor, placenta previa, and low-lying placenta. An

MRI may be helpful in distinguishing between some of these entities if the

ultrasound and β-hCG measurements are not clear.

Table 32-6 Ultrasound Criteria for Cervical Pregnancy

19441. Gestational sac or placental tissue visualized within the cervix

2. Cardiac motion noted below the level of the internal os

3. No intrauterine pregnancy

4. Hourglass uterine shape with ballooned cervical canal

5. No movement of the sac with pressure from transvaginal probe (i.e., no “sliding

sign” that is typically seen with incomplete abortions)

6. Closed internal os

Adapted from Kung FT, Lin H, Hsu TY, et al. Differential diagnosis of cervical ectopic

pregnancy and conservative treatment with the combination of laparoscopy-assisted uterine

artery ligation and hysteroscopic endocervical resection. Fertil Steril 2004;81:1642–1649.

Management. Options for management of cervical ectopic pregnancies

include medical treatment with methotrexate and surgical dilation and

curettage. The ideal regimen for medical management is unknown and success is

reported with both the single- and multidose regimens. More advanced gestations,

especially with fetal cardiac activity, may require a combination of multidose

methotrexate and intra-amniotic/intra-fetal injection of KCl for fetal demise.

These injections require skill to avoid rupture of membranes during the

procedure. A retrospective study of 62 women with cervical ectopic pregnancies

treated in China found that viable pregnancies treated with methotrexate had a

higher rate of surgical intervention (43%) than nonviable pregnancies (13%)

(130). As this was a retrospective chart review, the authors do not comment on

whether some of the additional procedures were planned or if they were a result

of methotrexate failure. As with tubal ectopic pregnancies, medical management

is appropriate only for those patients who are hemodynamically stable (129).

Preventative surgical management of cervical ectopic pregnancy has been

described. If the patient and physician elect to proceed with surgical management,

the preoperative preparation should include blood typing and cross-matching,

establishment of intravenous access, and detailed informed consent. This consent

should include the possibility of hemorrhage that may require transfusion or

hysterectomy. In a small study of 6 patients with cervical ectopic in China, all

patients were treated with uterine artery embolism (UAE) followed by

hysteroscopic endocervical resection (129). Treatment was successful in all six

cases without any adjunctive methotrexate treatment and with minimal blood loss

(mean 125 mL, range 50 to 250 mL). While this study is small, it suggests the

safety of minimally invasive techniques. Patients should be counseled that the

1945fertility effects of undergoing even a temporary UAE are not yet clear.

When a cervical ectopic pregnancy presents with bleeding or if bleeding occurs

as part of treatment, various techniques can be used including uterine packing,

UAE, lateral cervical suture placement to ligate the lateral cervical vessels,

placement of a cerclage, and insertion of an intracervical 30-mL Foley catheter

for cervical tamponade. When none of these methods is successful, hysterectomy

is required.

Ovarian Pregnancy

Diagnosis. A pregnancy confined to the ovary accounts for up to 3% of all

ectopic pregnancies and is the most common type of nontubal ectopic

pregnancy (1,131). Though the clinical presentation of bleeding, abdominal pain,

and positive pregnancy test is similar, ovarian pregnancy is not associated with

PID, infertility, or tubal disease like other ectopic pregnancies (132). The

pathology diagnostic criteria were described in 1878 by Spiegelberg, listed in

Table 32-7 (133). Ultrasound criteria are difficult as findings are typically a

cystic ovarian mass, with a differential of corpus luteum cyst, hemorrhagic cyst,

and tubal ectopic pregnancy.

Management. The treatment of ovarian pregnancy has changed. Whereas

oophorectomy was advocated in the past, ovarian cystectomy and/or wedge

resection is utilized with success and successful treatment with methotrexate has

been reported (134–136).

Abdominal Pregnancy

Abdominal pregnancies are one of the rarest types of ectopic pregnancy,

representing 1% to 1.4% of ectopic pregnancies (1,131). They are typically

classified as primary or secondary. Pathology criteria for primary abdominal

ectopic pregnancy have been established by Studdiford (see Table 32-8) and

include normal tubes and ovaries, no evidence of uteroplacental fistula (137).

Secondary abdominal pregnancies are more common, thought to result from tubal

abortion or rupture or, less often, from subsequent implantation within the

abdomen after uterine rupture. Risk factors for abdominal pregnancy include PID,

multiparity, endometriosis, assisted reproductive techniques, and tubal damage.

The most common area of implantation within the abdomen is the posterior culde-sac, and pregnancies have been confirmed in the mesosalpinx, omentum and

bowel, liver, spleen, abdominal wall, and within broad ligament (131).

Abdominal pregnancy is associated with high morbidity and mortality, with

the risk for death seven to eight times greater than from tubal ectopic

pregnancy and 50 times greater than from intrauterine pregnancy, most

likely resulting from later diagnosis.

1946Table 32-7 Criteria for Ovarian Pregnancy Diagnosis

1. The fallopian tube on the affected side must be intact

2. The fetal sac must occupy the position of the ovary

3. The ovary must be connected to the uterus by the ovarian ligament

4. Ovarian tissue must be located in the sac wall

From Spiegelberg O. Casusistik der ovarialschwangerschaft. Arch Gynaecol 1878;13:73.

Table 32-8 Studdiford’s Criteria for Diagnosis of Primary Abdominal Pregnancy

1. Presence of normal tubes and ovaries with no evidence of recent or past pregnancy

2. No evidence of uteroplacental fistula

3. The presence of a pregnancy related exclusively to the peritoneal surface and early

enough to eliminate the possibility of secondary implantation after primary tubal

nidation

Adapted from Anderson PM, Opfer EK, Busch JM, et al. An early abdominal wall

ectopic pregnancy successfully treated with ultrasound guided intralesional methotrexate:

A case report. Obstet Gynecol Int 2009; Article ID 247452.

Diagnosis. Ultrasound detects only approximately half of all abdominal ectopic

pregnancies. Ultrasound features that have been suggested include gestational sac

surrounded by loops of bowel, normal-appearing fallopian tubes, and ovaries

(138). Differential includes intrauterine pregnancy with retroflexion or distorted

by fibroids. When diagnosis is highly suspected, MRI may be helpful in assessing

degree of vascular attachment to other abdominopelvic tissues. Abdominal

ectopic pregnancies are one of the few ectopic pregnancies that may proceed out

of the first trimester. In advanced abdominal pregnancy, the clinical presentation

may include painful fetal movement, fetal movements high in the abdomen or

sudden cessation of movements. Physical examination may disclose persistent

abnormal fetal positioning, abdominal tenderness, a displaced uterine cervix, easy

palpation of fetal parts, and palpation of the uterus separate from the gestation.

The diagnosis may be suspected when there are no uterine contractions after

oxytocin infusion.

Management. Because the pregnancy can continue to term, the potential

maternal morbidity and mortality are very high. As a result, surgical intervention

1947is recommended when an abdominal pregnancy is diagnosed. At surgery, the

placenta can be removed if its vascular supply can be identified and ligated, but

hemorrhage can occur, requiring abdominal packing that is left in place and

removed after 24 to 48 hours. Options for management of hemorrhage include

artery embolization. If the vascular supply cannot be identified, the cord is ligated

near the placental base, and the placenta is left in place. Placental involution can

be monitored using serial ultrasonography and assessment of β-hCG levels.

Potential complications of leaving the placenta in place include bowel

obstruction, fistula formation and sepsis as the tissue degenerates. There are

concerns regarding the use of methotrexate treatment in abdominal pregnancies.

Specifically, there is theoretically an increased risk of infection and sepsis

resulting from the rapid tissue necrosis that occur following methotrexate

administration. There are reports of successful treatment of abdominal

pregnancies with methotrexate in patients not considered to be optimal surgical

candidates (136,139). There are scattered reports of term abdominal pregnancies.

When this occurs, perinatal morbidity and mortality are high, usually as a result

of growth restriction and congenital anomalies such as fetal pulmonary

hypoplasia, pressure deformities, and facial and limb asymmetry (140).

Interstitial Pregnancy

Diagnosis. Interstitial pregnancies represent about 2.4% of ectopic

pregnancies (1). This section of the fallopian tube is relatively thick with an

increased capacity to expand prior to rupture. This ability along with increased

vascularity of this area may allow these types of ectopic pregnancies to remain

asymptomatic for 7 to 16 weeks of gestation (141). However, late presentations

are rare; most patients typically present between 6 and 8 weeks of gestation (141).

Differential diagnosis includes angular pregnancy, a viable pregnancy that

implants within the uterine angle, medial to the ostia. Typically angular

pregnancies are asymptomatic unless they end in miscarriages, which occurs

approximately 38% of the time (141). In laparoscopy, these can be distinguished

as a true interstitial pregnancy appears lateral to the round ligament.

Interstitial pregnancies represent a disproportionately large percentage of

fatalities from ectopic pregnancy with a 2.5% mortality rate (142).

Management. These pregnancies were classically treated with laparotomy with

cornual resection. Early detection allows for a more conservative management

approach in hemodynamically stable patients without evidence of rupture.

Medical management with methotrexate is well described, with the single- and

multidose regimens. Approximately 10% to 20% of patients treated medically

will ultimately require surgery and close follow-up is warranted as with all

medically managed ectopic pregnancies (141).

1948Although cornual wedge resection by laparotomy is an acceptable surgical

option, minimally invasive techniques exist, including cornual excision,

minicornual excision and cornuostomy. Laparoscopic approaches are more

widely used and are dependent on surgical skill. Transcervical suction evacuation

under laparoscopic or ultrasound guidance has been reported (136,141). The

appropriate surgical technique and approach depends on the individual patient

presentation and the surgeon’s expertise.

Cesarean Scar Ectopic Pregnancy

A pregnancy implanted within the uterine myometrium at the site of a healed

cesarean scar is called a cesarean scar pregnancy (CSP). Historically, CSP has

been an extremely rare occurrence. However, as cesarean deliveries are becoming

more common, incidence has been slowly increasing with estimations among

women with prior cesarean deliveries to be up to 1:500 pregnancies (136,143).

Because of small numbers, it has not been determined if the number of cesarean

deliveries or type of cesarean delivery (e.g., classical or Pfannenstiel incisions)

affect risk of CSP.

Diagnosis. Ultrasound is the initial diagnostic tool for CSP, with signs

suggestive of CSP including: (1) gestational sac located at the level of the prior

scar; (2) surrounding Doppler flow with minimal separation from bladder; (3)

outward bulging of the gestational sac within the scar; and (4) negative “sliding

organs sign” where the gestational sac does not move with gentle pressure from

transvaginal ultrasound (143). CSPs have been separated into two types based on

prognosis; type 1 progressing toward uterine cavity; type 2 progressing toward

the bladder.

Management. As CSP is quite rare and can present differently based on

location and type, there is no management that has proved superior. Early

treatment is recommended, as CSP can progress into placental accreta or even

uterine rupture if left untreated. Management options include medical

management with systemic or local injection of either methotrexate or KCl,

surgical management with dilation and curettage with or without hysteroscopy,

UAE, or some combination of these approaches. Medical management should

only be offered to hemodynamically stable patients. Success rates for

methotrexate doses systemically, locally, and combined systemic/local have been

reported as 56%, 60%, and 77%, respectively. These cases had a hemorrhage rate

of 7% and hysterectomy rate of 3% (143). For surgical management, dilation and

curettage under ultrasound guidance had a reported success rate of 76% with a

30% hemorrhage risk and 3% hysterectomy risk (143). Hysteroscopic resection

was used with a 62% risk of persistent trophoblastic disease and should be

performed in combination with dilation and curettage. A combination of

1949methotrexate followed by dilation and curettage has been reported with success

rates up to 86%, hemorrhage risk of 14%, and hysterectomy risk of 4%. UAE can

be performed prior to dilation and curettage or even prior to methotrexate therapy

to reduce hemorrhage risk. If UAE is not done prophylactically, it can be done in

the case of hemorrhage after other treatment options. In some cases, methotrexate

was injected into uterine arteries prior to UAE. If used alone, UAE has a reported

success rate of 81% with risk of hemorrhage 5%, and risk of hysterectomy 4%

(143).

Heterotopic Pregnancy

Heterotopic pregnancy occurs when intrauterine and ectopic pregnancies

coexist. The reported incidence is 1:30,000 (35). The diagnosis is often delayed

as an intrauterine pregnancy is seen during ultrasonography examination and an

extrauterine pregnancy may be overlooked. Patients undergoing ovulation

induction with assisted reproductive technology are at risk of ovarian

hyperstimulation syndrome (OHSS), which can cause similar ultrasound findings

including enlarged ovaries and free fluid in the abdomen and is important to

consider in differential diagnosis (144). Serial β-hCG levels are not helpful

because the intrauterine pregnancy causes the β-hCG level to rise appropriately.

Heterotopic pregnancy is much more common with assisted reproductive

technology than spontaneous conception, with possible etiologies including

multiple embryo transfers and previous tubal damage (144,145).

Management. The ectopic pregnancy is treated surgically if the intrauterine

pregnancy is desired. When the ectopic pregnancy is removed, the intrauterine

pregnancy continues in most patients. The rate of spontaneous abortion is higher

with approximately one in three ending in miscarriage (145,146). It may be

possible to treat the ectopic pregnancy using nonchemotherapeutic medical

treatment, such as KCl, by transvaginal or laparoscopically directed injection;

however, a reported 55% may require additional surgical treatment (147).

Multiple Ectopic Pregnancies

Twin or multiple ectopic gestations occur less frequently than heterotopic

gestations and may appear in a variety of locations and combinations.

Multiple ectopic pregnancies are thought to be rare, but with the advent of

assisted reproductive technologies the incidence appears to be rising. A

review of bilateral tubal pregnancies reported 242 cases between 1918 and 2007,

with 42 cases in the past 10 years alone. Fifty percent of these twin tubal

pregnancies were associated with assisted reproductive technologies (148).

Another review of 163 cases of tubal ectopic pregnancies had a reported rate of

twin tubal pregnancies of 2.4% (149). Although most reports are confined to twin

1950tubal gestations, ovarian, interstitial, and abdominal twin pregnancies have been

reported. Management is similar to that of other types of ectopic pregnancy and is

somewhat dependent on the location of the pregnancy.

Pregnancy After Hysterectomy

The most unusual form of ectopic pregnancy is one that occurs after vaginal or

abdominal hysterectomy (150,151). Such a pregnancy may occur after

supracervical hysterectomy because the patient has a cervical canal that may

provide intraperitoneal access (149). Pregnancy may occur in the perioperative

period with implantation of the already fertilized ovum in the fallopian tube.

Pregnancy after total hysterectomy probably occurs secondary to a vaginal

mucosal defect that allows sperm into the abdominal cavity. Only case reports are

available and management options include surgical removal and systemic

methotrexate, depending on location of pregnancy and patient characteristics.

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