Chapter 20. Antepartum Fetal Assessment. Will Obs

 Chapter 20. Antepartum Fetal Assessment

BS. Nguyễn Hồng Anh

Techniques employed to evaluate fetal health focus on fetal biophysical ndings that include heart rate, movement, breathing,

and amnionic uid production. Antepartum etal surveillance

aims to prevent etal death in pregnancies with complex maternal and etal conditions yet avoid unnecessary interventions

(American College of Obstetricians and Gynecologists, 2021a).

Most etuses will be healthy, and a normal antepartum test

result is highly reassuring. Fetal death within 1 week o a normal test result is rare. Indeed, negative predictive values—true

negative test results for fetal jeopardy—for most of the tests

described are 99.8 percent or higher. In contrast, estimates o

positive predictive values—true positive test results for fetal

jeopardy—are low and range between 10 and 40 percent. e

benet of fetal surveillance is primarily based on circumstantial evidence. No denitive randomized clinical trials have been

conducted or obvious ethical reasons (American College o

Obstetricians and Gynecologists, 2021a).

FETAL MOVEMENTS

■ Fetal Behavioral States

Fetal activity commences as early as 7 weeks’ gestation (Sajapala, 2017; Vindla, 1995). Between 20 and 30 weeks’ gestation, general body movements become organized, and the

etus starts to show rest-activity cycles (Sorokin, 1982). Tese

cycles reect central nervous system development and maturation. By approximately 36 weeks’ gestation, rest-activity cycles

give way to behavioral states in most normal etuses (Peirano,

2003). Four etal behavioral states are described by Nijhuis and

coworkers (1982):

• State 1F is a quiescent state—quiet sleep—with a narrow

oscillatory bandwidth o the etal heart rate.

• State 2F includes frequent gross body movements, continuous eye movements, and wider oscillation o the etal heart

rate. Tis state is analogous to rapid eye movement (REM)

or active sleep in the neonate.

• State 3F includes continuous eye movements in the absence

o body movements and no heart rate accelerations. Tis

state is rare, and its existence is disputed (Pillai, 1990a).

• State 4F is one of vigorous body movement with continuous

eye movements and heart rate accelerations. Tis state corresponds to the awake state in newborns.

At 28 to 30 weeks’ gestation, etuses transition to spend

most of their time in states 1F and 2F, namely, in quiet or

active sleep (Fig. 20-1) (Peirano 2003; Suwanrath, 2010). For

example, at 38 weeks, 75 percent o time is spent in these two

states. Tese behavioral states have been used to develop an

increasingly sophisticated understanding o etal behavior. In

a study o etal urine production, bladder volumes rose during state 1F quiet sleep and signicantly declined during state

2F active sleep due to diminished urine production and inrequent fetal voiding (Oosterhof, 1993). ese phenomena were

thought to represent reduced renal blood ow during active

sleep.

■ Determinants of Fetal Activity

One determinant of fetal activity is the just-described sleepawake cycles, which are independent o maternal ones. In one

study o 16 near-term etuses, the mean duration o a complete

cycle, which included quiet and active states, was 60 minutes. e

mean duration was 23 minutes for the quiet states and 40 minutes

or the active states (imor-ritsch, 1978). Patrick and associates (1982) measured gross etal body movements with real-time

sonography for 24-hour periods in 31 normal near-term pregnancies and ound the longest period o inactivity to be 75 minutes.

Amnionic uid volume is another actor afecting etal activity. Sherer and colleagues (1996) assessed the number o etal

movements in relation to amnionic uid volume in 465 preterm pregnancies during biophysical prole testing. Fetal

activity declined in those with diminished amnionic volumes,

and the authors suggested that a restricted uterine space might

physically limit etal movements.

Patient habits and medications alter etal movement. For

example, maternal smoking decreases etal activity (Coppens,

2001; Taler, 1980). reatment o substance abuse disorders

with methadone and buprenorphine also reduces etal movement

(Jansson, 2017; Wouldes, 2004). Betamethasone administration

is associated with decreased fetal movement for 24 to 72 hours,

and the diurnal pattern is lost (Koenen, 2005; Mulder, 2009).

Te efect is less clear with dexamethasone. Maternal acetaminophen ingestion does not alter etal movement, but a glucose load

does promote activity (Aladjem, 1979; Nitsche, 2015).

■ Maternal Perception

Sadovsky and coworkers (1979b) classied fetal movements as

weak, strong, and rolling according to maternal perceptions

and independent recordings using piezoelectric sensors. As

pregnancy advances, the rate o weak movements drops, and

more vigorous ones rise in frequency. e latter then subside at

term. Presumably, declining amnionic uid volume and space

account or diminished activity at term. Figure 20-2 shows

etal movements during the last hal o gestation in 127 pregnancies with normal outcomes. Te mean number o weekly

movements calculated rom 12-hour daily recording periods

rose rom approximately 200 at 20 weeks’ gestation to a maximum o 575 movements at 32 weeks. Weekly etal movement

counts then declined to an average of 282 at 40 weeks. Another

study o nearly 300 gravidas ound a similar movement pattern

across gestational ages (Bradord, 2019b). More than 90 percent o these women reported stronger movements during the

evening and nighttime.

Women perceive 16 to 45 percent of fetal movements

detected by sonography (Brown, 2016). A higher body mass

index does not decrease maternal perception o etal movement

(Bradord, 2019a; Sasson, 2016). It remains unclear i nulliparity or anterior placental location afect maternal impression o

etal activity (Brown, 2016; Sasson, 2016).

■ Clinical Application

Because women may perceive a decline in etal movement in

the days to weeks preceding stillbirth, maternal perception o

etal movement has been evaluated as a preventive aid (Heazell,

2008; Stacey, 2011). However, the optimal etal-movement

protocol remains undened (Mangesi, 2015). Some methods

rely on quantitative counts, such as 10 movements in 2 hours,

whereas others rely on a mother's subjective impression. In one

study, more than 68,000 pregnancies were randomly assigned

between 28 and 32 weeks’ gestation to an objective or subjective assessment group (Grant, 1989). Women in the objective

arm were instructed to record the time needed to eel 10 movements each day. is required an average of 2.7 hours daily.

Women in the subjective group were inormally asked about

movements during prenatal visits. Reports o decreased etal

motion were then evaluated with tests o etal well-being. Antepartum death rates or normal singleton etuses were similar

in the two study groups, and most stillborn etuses were dead

by the time the mothers reported or medical attention. Rather

than concluding that maternal perceptions were meaningless,

the authors concluded that inormal maternal impressions were

as valid as ormally recorded etal movement counts.

Women's knowledge o these protocols also vary. In one

survey of more than 400 gravidas, 85 percent noted receiving

30–31 32–33 34–35 36–37 38–40

100

0

Gestational age (wks)

20

40

60

Number of sleep minutes

Number of accelerations

80

100

0

20

40

60

80

Active sleep

Active sleep acceleration

Quiet sleep acceleration

Quiet sleep

FIGURE 20-1 The change in fetal sleep patterns (blue columns)

and corresponding number of accelerations (graph lines). As the

third trimester advances, fetuses spend more time in active sleep

and the number of accelerations per hour increases.

18

Gestational age (weeks)

Fetal movements

(weekly average)

600

500

400

300

200

100

0

20 22 24 26 28 30 32 34 36 38 40

FIGURE 20-2 Graph depicts averages of fetal movements

counted during 12-hour periods (mean ± SEM). (Data from

Sadovsky, 1979a.)Antepartum Fetal Assessment 385

CHAPTER 20

instruction rom their provider regarding normal etal movement (Pollock, 2019). However, 60 percent described it as a

subjective assessment, and 40 percent reported a quantitative

method. In another study, more than two thirds o women

could not explain fetal movement monitoring techniques

(Berndl, 2013).

Although a long-standing pillar o maternal care, etal movement surveillance may not be as predictive as thought. Nearly

6 to 7 percent o pregnancies are complicated by decreased

etal movement, but stillbirth rates are not increased in these

cases (Harrington, 1998; Scala, 2015). In one study o more

than 400,000 pregnancies complicated by decreased fetal

movement, directed patient and staf education coupled with

a prescriptive management plan did not reduce the stillbirth

incidence (Norman, 2018). Aside rom stillbirth, some data

show that decreased etal movement may help identiy growthrestricted etuses beore birth (Saastad, 2011; Scala, 2015). In

one o these studies, nearly 1100 pregnancies were assigned to

subjective or objective assessment o etal activity. Te rate o

1-minute Apgar scores ≤3 was signicantly reduced (0.4 versus

2.3 percent) when counting was used (Saastad, 2011).

Tus, although most pregnancies complicated by decreased

etal movement will result in normal outcomes, maternal perception o reduced etal activity warrants urther evaluation.

No consensus guides a provider's response. Care is individualized and inuenced by gestational age and pregnancy comorbidities. In low-risk pregnancies, documenting etal heart tones

and adequate amnionic uid volume may be sucient. For

older etuses, tests described in the upcoming sections are typically added. Our practice is a nonstress test and sonographic

measurement o amnionic uid volume.

Many women report excessive etal movement in the third

trimester. Its risk, i any, is poorly understood. Stacey and colleagues (2011) reported that a single episode o vigorous etal

activity is associated with a greater risk or etal death. Similarly,

others have described maternal perception o a single episode

o excessive o etal movement in the weeks prior to a stillbirth

diagnosis (Heazell, 2017; Whitehead, 2020). More research is

needed prior to recommending intervention.

FETAL BREATHING

Small inward and outward ow o tracheal uid, indicating

thoracic movement, was rst identied in fetal sheep (Dawes,

1972). Tese chest wall movements difer rom those ollowing birth in that they are discontinuous. Another eature o

etal respiration is paradoxical chest wall movement (Fig. 20-3)

( Johnson, 1988). In the newborn or adult, the opposite occurs.

One interpretation of paradoxical respiratory motion might

be coughing to clear amnionic uid debris. Although the

physiological basis or the breathing reex is not completely

understood, such exchange o amnionic uid appears to be

essential for normal lung development (Chap. 7, p. 130). Dawes

(1974) identied two types of respiratory movements. e rst

are gasps or sighs, which occurred at a frequency of 1 to 4 per

minute. Te second, irregular bursts o breathing, occurred at

rates up to 240 cycles per minute. ese latter rapid respiratory

movements were associated with rapid eye movement. Badalian

and associates (1993) studied the maturation o normal etal

breathing using color ow and spectral Doppler analysis of

nasal uid ow as an index o lung unction. Tey suggested

that etal respiratory rate declined in conjunction with increasing respiratory volume at 33 to 36 weeks’ gestation and coincided with lung maturation.

Many investigators have used sonography to determine

whether chest wall movements might reect etal health. Several

variables in addition to hypoxia afect these movements. Tese

include maternal hypoglycemia, sound stimuli, cigarette smoking, amniocentesis, impending preterm labor, gestational age,

fetal heart rate, and labor—during which it is normal for fetal

respiration to cease.

Because etal breathing movements are episodic, interpretation o etal health when respirations are absent may be di-

cult. Patrick and coworkers (1980) performed continuous

24-hour observation using sonography to characterize fetal

breathing patterns during the last 10 weeks o pregnancy. A

total of 1224 hours of fetal observation in 51 pregnancies were

collected. Figure 20-4 displays the percentages o time spent

breathing near term. Substantively diminished breathing during the night suggests a diurnal pattern. In addition, breathing

activity is enhanced somewhat ollowing maternal meals. otal

absence o breathing was observed in some o these normal

etuses or up to 122 minutes, indicating that etal evaluation

to diagnose absent respiratory motion may require long periods

o observation.

Te potential or breathing activity to be an important sole

marker of fetal health is unfullled because of the multiple

Expiration

B Expiration

Fetal

chest

Fetal

abdomen

A

Inspiration

Fetal

chest

Fetal

abdomen

FIGURE 20-3 Paradoxical chest movement with fetal respiration.

During inspiration (A), the chest wall paradoxically collapses and

the abdomen protrudes, whereas during expiration (B), the chest

wall expands.386 The Fetal Patient

Section 6

actors that afect breathing. Most clinical applications couple

etal breathing with other biophysical indices. For example,

fetal breathing is one component of the biophysical prole

(p. 389).

CONTRACTION STRESS TEST

During a uterine contraction, pressures generated by the myometrium exceed the collapsing pressure o the vessels coursing

through it. Tis ultimately lowers blood ow to the placenta's

intervillous space. Brie periods o impaired oxygen exchange

result. I uteroplacental pathology is present, oxygen exchange

to the etus is urther diminished, and late etal heart rate decelerations appear (Chap. 24, p. 452). ese downward- sloping

heart-rate waveorms begin with the onset o the uterine

contraction waveorm or just beyond its acme. Instead, contractions may produce variable decelerations as a result o cord

compression. Tis suggests oligohydramnios, which is oten

comorbid with placental insuciency.

Ray and associates (1972) used this concept in 66 complicated pregnancies and developed the oxytocin challenge test,

which was later called the contraction stress test (CST). A positive test result, that is, an abnormal result, is uniorm repetitive

late etal heart rate decelerations. In their study, the tests were

repeated weekly, and the authors concluded that negative CS

results, that is, normal results, orecasted etal health.

o perorm the test, contractions are induced with either

intravenous oxytocin or nipple stimulation. I at least three

spontaneous contractions of 40 seconds or longer are present in a 10-minute span, no uterine stimulation is necessary

(American College of Obstetricians and Gynecologists, 2021a).

Te etal heart rate and uterine contractions are recorded simultaneously by external monitors. With oxytocin, a dilute intravenous inusion is used to establish a satisactory contraction

pattern (Freeman, 1975). At Parkland Hospital, 20 units o

oxytocin are mixed in 1 liter o Ringer solution and initiated at

a rate o 6 mU/min. Te rate is increased by 6 mU/min every

40 minutes to achieve the just-discussed contraction pattern.

Nipple stimulation to induce uterine contractions or a CS

is usually successful (Huddleston, 1984). One method involves

a woman rubbing one nipple through her clothing or 2 minutes or until a contraction begins. Tis 2-minute nipple stimulation ideally will induce a pattern o three contractions per

10 minutes. I not, ater a 5-minute rest interval, she is instructed

to retry nipple stimulation to achieve the desired pattern. I this

is unsuccessul, dilute oxytocin may be used.

CS results are interpreted according to the criteria shown

in Table 20-1. Disadvantageously, the average CST requires

90 minutes to complete. Compared with oxytocin, nipple stimulation shortens testing time and costs less. During a CST, some

have reported unpredictable uterine hyperstimulation and etal

distress, whereas others did not nd excessive activity to be harmful

(Frager, 1987; Schellpefer, 1985). Relative contraindications to a

CS include those conditions that contraindicate vaginal delivery.

NONSTRESS TEST

Te nonstress test (NST) also is used to assess etal well-being

and employs the phenomenon o etal heart rate acceleration

in response to etal movement (Freeman, 1975; Lee, 1975).

During an NST, these accelerations are correlated with fetal

movements perceived by the mother.

Compared with a CS, an NS is easier to perorm, and normal results can be used to further dene false-positive CST results.

Simplistically, the NS is primarily a test o etal condition, and it

difers rom the CS, which is considered a test o uteroplacental

unction. Currently, NS is the most widely used primary testing

method or assessment o etal well-being. It has also been incorporated into the biophysical prole, discussed later (p. 389).

TABLE 20-1. Criteria for Interpretation of the Contraction Stress Test

Negative: no late or significant variable decelerations

Positive: late decelerations following 50% or more of contractions (even if the contraction frequency is fewer than three in

10 minutes)

Equivocal-suspicious: intermittent late decelerations or significant variable decelerations

Equivocal-hyperstimulatory: fetal heart rate decelerations that occur in the presence of contractions more frequent than

every 2 minutes or lasting longer than 90 seconds

Unsatisfactory: fewer than three contractions in 10 minutes or an uninterpretable tracing

50

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10

8 am Noon 4 pm 8 pm Midnight

4 am 8 am

Time spent breathing (percent)

Time of day

FIGURE 20-4 The percentage of time spent breathing by 11

fetuses at 38 to 39 weeks’ gestation. Fetal breathing activity

significantly increases after breakfast. Breathing activity diminished

during the day and reached its minimum between 20:00 and

24:00 hours. The percentage of time spent breathing rose significantly between 04:00 and 07:00 hours, when mothers were asleep.

(Adapted with permission from Patrick J, Campbell K, Carmichael L,

et al: Patterns of human fetal breathing during the last 10 weeks of

pregnancy, Obstet Gynecol. 1980 Jul;56(1):24–30.)Antepartum Fetal Assessment 387

CHAPTER 20

■ Fetal Heart Rate Acceleration

Autonomic inuences are mediated by sympathetic or parasympathetic impulses rom brainstem centers to normally raise

or slow the etal heart rate. Te NS is based on the hypothesis

that the heart rate o a etus that is not acidemic as a result o

hypoxia or neurological depression will temporarily accelerate

in response to fetal movement (American College of Obstetricians and Gynecologists, 2019; Lee, 1975). Fetal movements

during testing are identied by maternal perception and selfrecorded. As hypoxia develops, etal heart rate accelerations

diminish (Smith, 1988).

Gestational age inuences acceleration of the fetal heart rate.

Pillai and James (1990b) studied patterns o etal heart rate

acceleration during normal pregnancy. Te percentage o body

movements accompanied by accelerations and the amplitude o

these waveorms both increase with gestational age (Fig. 20-5).

Accordingly, the National Institute o Child Health and

Human Development Fetal Monitoring Workshop dened

acceleration waveorms based on gestational age (Macones,

2008). In etuses at or beyond 32 weeks’ gestation, the acceleration acme is ≥15 beats per minute (bpm) above the baseline

rate, and the acceleration lasts ≥15 seconds but <2 minutes.

Before 32 weeks, accelerations are dened as having a rise

≥10 bpm above baseline or ≥10 seconds. In one study o 188

nor mal etuses at 25 and 28 weeks’ gestation, only 70 percent

demonstrated the required ≥15 bpm during heart rate accelerations. Accelerations of 10 bpm occurred in 90 percent (Guinn,

1998). Cousins and associates (2012) compared the Workshop criteria recommended beore 32 weeks with the standard

15 bpm/15 second criteria in a randomized trial of 143 women.

Tey ound no diferences in perinatal outcomes.

■ Reactive (Normal) Nonstress Tests

A normal NS is termed reactive and requires two or more

accelerations within 20 minutes o beginning the test (Fig. 20-6)

(American College of Obstetricians and Gynecologists, 2021a).

Accelerations are accepted irrespective o etal movement. Beore

concluding that a test is nonreactive, a 40-minute or longer tracing should be perormed. Tis threshold accounts or etal sleep

cycles (Paul, 1995). Miller and coworkers (1996b) reviewed outcomes in etuses with NS results that were considered nonreactive because only one acceleration was recorded. Tey concluded

that one acceleration was as reliable as two in predicting healthy

etal status. NSs are more likely to be reactive and have a

shorter testing time in the evening compared with the morning

(Babazadeh, 2005; Petrikovsky, 1996).

Loud external sounds have been used to startle the etus

and thereby provoke heart rate acceleration. A commercially

available acoustic stimulator is positioned on the maternal

abdomen, and a stimulus o 1 to 2 seconds is applied (Eller,

1995). Tis may be repeated up to three times or up to 3

seconds (American College of Obstetricians and Gynecologists, 2021a). In a randomized trial o 113 women undergoing NS, vibroacoustic stimulation lowered the average

testing time and incidence o nonreactive test results (PerezDelboy, 2002; Turitz, 2012). One case report described a

etal tachyarrhythmia that was provoked with vibroacoustic

stimulation in a etus with known premature atrial contractions (Laventhal, 2003).

Although a normal number and amplitude o accelerations seems to reect etal well-being, their absence does not

100

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60

50

40

30

20

10

0

16 20 24 28 32 36 40

Fetuses with accelerations (percent)

Weeks’ gestation

FIGURE 20-5 Percentage of fetuses with at least one acceleration

of 15 bpm sustained for 15 seconds concurrent with fetal movement. (Redrawn from Pillai M, James D: The development of fetal

heart rate patterns during normal pregnancy, Obstet Gynecol. 1990

Nov;76(5 Pt 1):812–816.)

FIGURE 20-6 Reactive nonstress test. Notice there are at least two fetal heart rate accelerations (arrows) of more than 15 beats/min for

longer than 15 seconds. The black line reflects fetal heart rate, whereas the purple line reflects the mother’s.388 The Fetal Patient

Section 6

invariably predict etal compromise.

Indeed, some investigators have reported

alse-positive rates ≥90 percent (Devoe,

1986). Because healthy etuses may not

move or up to 75 minutes, some have

considered that a longer NS duration might increase the positive predictive value o the NS (Brown, 1981).

In this scheme, either the test became

reactive during a period up to 80 minutes or the test remained nonreactive or

120 minutes, which indicated a very ill

etus. Tereore, prolonged etal heart

rate monitoring is undertaken i an NS

remains nonreactive after 40 minutes.

■ Nonreactive (Abnormal)

Nonstress Tests

Based on the oregoing, a nonreactive

NS is not always ominous and can

be seen with a sleeping etus. An NS

result can also revert to normal as the

etal condition changes. An example is

shown in Figure 20-7. Instead, a reactive NS result can become abnormal i

the etal condition deteriorates.

Some abnormal patterns reliably orecast severe etal jeopardy (Fig. 20-8). For

example, Devoe and colleagues (1985)

concluded that >90 percent o NS

results that were nonreactive or 90 minutes or more were associated with signi-

icant perinatal pathology. Specically,

Visser and associates (1980) described a

terminal cardiotocogram, which included:

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A B

FIGURE 20-7 Two antepartum fetal heart rate (FHR) tracings in a 28-week pregnant

woman with diabetic ketoacidosis. A. FHR tracing (upper panel) and accompanying

contraction tracing (second panel). Tracing, obtained during maternal and fetal acidemia, shows absence of accelerations, diminished variability, and late decelerations with

weak spontaneous contractions. B. Fetal heart rate tracing shows return of normal

accelerations and variability of the fetal heart rate following correction of maternal

acidemia.

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Nonstress Test

45 minute mark

Time: 0415 hr

119 91

Oxytocin Challenge Test

Dosage: 8 mU/min

Time: 0615 hr

Cesarean Section: 0650 hr

FHR 110 BPM

Apgar 1/0

Umbilical vein pH = 6.58

FIGURE 20-8 Nonreactive nonstress test (left side of tracing) followed by contraction stress test showing mild, late decelerations (right side

of tracing). Cesarean delivery was performed, and the severely acidemic fetus could not be resuscitated.Antepartum Fetal Assessment 389

CHAPTER 20

(1) baseline variability <5 bpm, (2) absent accelerations, and (3)

late decelerations with spontaneous uterine contractions. Tese

mirrored experiences rom Parkland Hospital in which absence o

accelerations during an 80-minute recording period in 27 etuses

was associated consistently with evidence o uteroplacental pathology (Leveno, 1983). Te latter included etal-growth restriction

in 75 percent, oligohydramnios in 80 percent, etal acidemia in

40 percent, meconium in 30 percent, and placental infarction in

93 percent.

■ Interval Between Testing

Set originally and arbitrarily at 7 days, the interval between

NSs varies based on indication (Paul, 1995). According to the

American College of Obstetricians and Gynecologists (2021a),

weekly testing is undertaken in the setting o stable maternal

medical conditions such as pregestational diabetes, chronic

hypertension, or lupus. For high-risk conditions such as preeclampsia remote rom term, some perorm NSs daily. Fetal

conditions require individualized periodic testing based on the

etal complication. Fetal-growth restriction is a common indication for which testing intervals vary (Fig. 47-6, p. 829).

■ Decelerations During Nonstress Testing

Fetal movements commonly produce heart rate decelerations.

In one study o 16 near-term etuses, one hal to two thirds

o NS tracings had decelerations, depending on the vigor o

the etal motion (imor-ritsch, 1978). Tis high incidence

o decelerations inevitably makes interpretation o their signicance problematic. Indeed, Meis and coworkers (1986)

reported that variable etal heart rate decelerations during

NSs were not a sign o etal compromise. Te American College of Obstetricians and Gynecologists (2021a) has concluded

that variable decelerations, if nonrepetitive and brief—less than

30 seconds—do not indicate fetal compromise or the need for

obstetrical intervention. Repetitive variable decelerations—at

least three in 20 minutes—have been associated with a greater

risk of cesarean delivery for fetal distress. Decelerations lasting

≥1 minute are reported to have an even worse etal prognosis

(Bourgeois, 1984; Druzin, 1981; Pazos, 1982).

Hoskins and associates (1991) attempted to rene interpretation o tests that showed variable decelerations by adding

sonographic estimation o amnionic uid volume. Te incidence

o cesarean delivery or intrapartum etal distress progressively

rose concurrently with the decline o amnionic uid volume.

Variable decelerations during an NS plus oligohydramnios

resulted in a 75-percent cesarean delivery rate. Fetal distress in

labor, however, also frequently developed in those pregnancies

with variable decelerations but with normal amounts o amnionic uid. Others report similar results (Grubb, 1992).

■ Falsereactive Nonstress Tests

In one review o etal death within 7 days o a reactive NS, the

most frequent indication for testing was postterm pregnancy

(Smith, 1987). Te mean interval between testing and death

was 4 days, with a range of 1 to 7 days. e single most common autopsy nding was meconium aspiration, often associated with some type o umbilical cord abnormality. Te authors

concluded that acute asphyxia had provoked etal gasping. Tey

considered an NST inadequate to preclude an acute asphyxial

event, but viewed amnionic uid volume assessment as valuable. Other ascribed frequent causes of fetal death despite a

alse-reactive NS included intrauterine inection, abnormal

cord position, etal malormations, and placental abruption.

BIOPHYSICAL PROFILE

Assessing ve specic fetal biophysical variables more accurately

predicts etal health (Manning, 1980). Tese include heart rate

acceleration, breathing, movement, tone, and amnionic uid

volume (Table 20-2). In the commonly used biophysical profle (BPP), each normal variable is assigned a score o 2, and

abnormal variables are given a score o 0. Tus, the highest

score possible or a normal etus is 10. Because etal breathing

and movement are episodic, 30 minutes are allotted to per-

orm a BPP beore a score o 0 is assigned to any component.

Figure 20-9 shows color Doppler evidence of amnionic uid

owing through the nares with etal breathing. In one study,

BPP scores were higher i a test was perormed in late evening

compared with the morning (Ozkaya, 2012). Narcotics and

sedatives can signicantly lower the score (Kopecky, 2000).

Using the BPP interpretation and management strategy

shown in Table 20-3, Manning and colleagues (1987) evaluated more than 19,000 pregnancies. Greater than 97 percent

o the tested pregnancies had normal results. Tey reported a

TABLE 20-2. Components and Scores for the Biophysical Profile

Component Score 2 Score 0

Nonstress testa ≥2 accelerations within 20–40 min 0 or 1 acceleration within 20–40 min

Fetal breathing ≥1 episode of rhythmic breathing lasting ≥30 sec <30 sec of breathing

Fetal movement ≥3 discrete body or limb movements <3 discrete movements

Fetal tone ≥1 episode of extremity extension and subsequent return

to flexion

0 extension/flexion events

Amnionic fluid

volumeb

A pocket of amnionic fluid that measures at least 2 cm in

two planes perpendicular to each other (2 × 2 cm pocket)

Deepest single vertical pocket ≤2 cm

aMay be omitted if all four sonographic components are normal.

bFurther evaluation warranted, regardless of biophysical composite score, if deepest vertical amnionic fluid pocket ≤2 cm.390 The Fetal Patient

Section 6

false-normal test rate—dened by an antepartum death of a

structurally normal fetus—that approximated 1 per 1000. e

most common identiable causes of fetal death after a normal

BPP score include etomaternal hemorrhage, umbilical cord

accident, and placental abruption (Dayal, 1999).

Subsequently, Manning and associates (1993) published a

remarkable description of 493 fetuses in which BPP scores were

correlated with umbilical venous blood pH values. Tis blood was

being obtained via antepartum cordocentesis for other specic

etal indications. Namely, karyotyping o etal-growth restricted

etuses constituted 20 percent, and the remainder had alloimmune hemolytic anemia requiring hemoglobin measurement.

As shown in Figure 20-10, a BPP score o 0 was almost invariably associated with signicant fetal acidemia, whereas a normal

score of 8 or 10 was associated with normal pH. An equivocal

test result—a score of 6—was a poor predictor of an abnormal

outcome. A drop in the BPP score from 2 or 4 down to 0 more

accurately predicted an abnormal fetal outcome. Overall, BPP

scores provided poor sensitivity to predict cord blood pH. Similar studies have concluded the same (Salvesan, 1993; Weiner,

1996). Kaur and colleagues (2008) perormed daily BPPs to

ascertain the optimal delivery time in 48 growth-restricted

preterm etuses that weighed <1000 g. Despite scores of 8 in

27 etuses and 6 in 13 others, 6 died and 21 were acidemic.

■ Modified Biophysical Profile

BPP performance is labor intensive and requires a trained

sonographer. In response, Clark and coworkers (1989) created

FIGURE 20-9 A sagittal color Doppler image displays the movement of amnionic fluid through the nares during fetal breathing.

TABLE 20-3. Interpretation of Biophysical Profile Score

Biophysical Profile Score Interpretation Recommended Management

10 Normal, nonasphyxiated fetus No fetal indication for intervention; repeat test weekly

8/10 (Normal AFV)

8/8 (NST not done)

Normal, nonasphyxiated fetus No fetal indication for intervention; repeat test weekly

8/10 (Decreased AFV) Chronic fetal asphyxia suspected If ≥36 weeks, deliver

If <36 weeks, monitor per institution's protocol

6 (Normal AFV) Equivocal If ≥37 weeks, deliver

If <37 weeks and normal fluid, repeat test in 24 hours

If repeat test >6, monitor per institution's protocol

6 (Decreased AFV) Possible fetal asphyxia If 36–37 weeks, deliver

If <36 weeks, monitor per institution's protocol

4 Probable fetal asphyxia If ≥32 weeks, deliver

If <32 weeks, individualize management based on maternal

and fetal conditions

0 or 2 Almost certain fetal asphyxia Deliver

AFV = amnionic fluid volume; NST = nonstress test.

Adapted from American College of Obstetricians and Gynecologists, 2021a; Liston, 2018; Manning, 2018.

7.40

7.35

7.30

7.25

7.20

7.10

7.05

10 8 6 4 2 0

*

*

*

*

Fetal biophysical profile score

Antepartum umbilical venous pH

FIGURE 20-10 Mean umbilical vein pH (±2 SD) obtained by

cordocentesis in relation to fetal biophysical profile score category.

(Data from Manning, 1993.)Antepartum Fetal Assessment 391

CHAPTER 20

an abbreviated BPP, in which an NS was coupled with amnionic uid volume assessment. In 2628 singleton pregnancies,

the NST was performed twice weekly. Described in the next

section, amnionic uid volume was measured by the amnionic

uid index (AFI), and values ≤5 cm were considered abnormal. is abbreviated BPP required approximately 10 minutes

to perorm. Te authors considered it a superb antepartum surveillance method because no etuses died unexpectedly.

From another study of 17,429 modied BPPs in 2774

women, investigators concluded that such testing was an

excellent fetal surveillance tool (Nageotte, 1994). Miller and

associates (1996a) reported results of more than 54,000 modi-

ed BPPs performed in 15,400 high-risk pregnancies. ey

described a alse-negative rate o 0.8 per 1000 and a alsepositive rate of 1.5 percent. e American College of Obstetricians and Gynecologists (2021a) has concluded that the BPP

and modied BPP are comparable to other biophysical fetal

surveillance approaches in predicting etal well-being.

AMNIONIC FLUID VOLUME

Te importance o amnionic uid volume estimation is indicated by its inclusion into virtually all schemes o etal health

assessment (Frøen, 2008). Physiologically, diminished uteroplacental perusion may lead to lower etal renal blood ow,

decreased urine production, and ultimately, oligohydramnios

(Chap. 14, p. 260). Amnionic uid volume is measured either

by the AFI or by the single deepest vertical pocket (DVP). In

one study o more than 1000 women in which both methods

were compared, AFI led to a higher rate o oligohydramnios

diagnoses and induction of labor. Despite this, perinatal outcomes were not improved (Kehl, 2016). Te American College of Obstetricians and Gynecologists (2021a) concludes that

DVP measurement, as opposed to AFI, is associated with fewer

unnecessary interventions but comparable perinatal outcomes

(Nabhan, 2008; Reddy, 2014). An AFI ≤5 cm and a DVP

≤2 cm is considered abnormal.

DOPPLER VELOCIMETRY

Blood ow velocity measured by Doppler ultrasound reects

downstream impedance (Chap. 14, p. 261). is Doppler

velocimetry has been used to interrogate the umbilical artery,

middle cerebral artery (MCA), and ductus venosus. With

umbilical artery studies, abnormal waveorms correlated with

placental villous hypovascularity. Specically, of the small placental arterial channels, 60 to 70 percent must be obliterated

before the umbilical artery Doppler waveform becomes abnormal. Such extensive placental vascular pathology has a major

efect on etal circulation. According to rudinger (2007),

because more than 40 percent of the combined fetal ventricular

output is directed to the placenta, obliteration o placental vascular channel increases aterload and leads to etal hypoxemia.

Tis in turn leads to ventricular dilation and redistribution o

MCA blood ow. Ultimately, pressure rises in the ductus venosus due to aterload in the right side o the etal heart (Baschat,

2004). Clinically, abnormal Doppler waveforms in the ductus

venosus are a late nding in the progression of fetal deterioration due to chronic hypoxemia. o predict placental dysunction, maternal uterine artery Doppler velocimetry also has been

assessed, with the goal to balance stillbirth against the risks o

preterm delivery (Ghidini, 2007).

■ Umbilical Artery

e umbilical artery systolic-diastolic (S/D) ratio is considered abnormal i it is above the 95th percentile or gestational

age or if diastolic ow is either absent or reversed (Chap. 14,

p. 262). Absent or reversed end-diastolic ow signies greater

impedance to umbilical artery blood ow (Fig. 47-7, p. 830).

It is reported to result rom poorly vascularized placental villi

and is seen in extreme cases o etal-growth restriction (odros,

1999). Te perinatal mortality rate is increased when absent or

reversed end-diastolic ow is present (Viero, 2004).

Umbilical artery Doppler velocimetry to evaluate fetal health

has been extensively assessed in randomized trials. In one, 1360

women with a high risk or stillbirth underwent either an NS

or Doppler velocimetry, and abnormal results prompted labor

induction. More patients in the Doppler group required induction, yet the cesarean delivery rate or intrapartum etal distress was 4.6 percent. is rate was signicantly lower than the

8.7-percent cesarean rate or those receiving an NS (Williams,

2003). e authors believed that Doppler assessment, compared with an NST, identied a higher proportion of patients

with early placental compromise. Identied early, these fetuses

experienced less distress, which yielded a lower cesarean delivery rate. Similarly, Yoon and coworkers (1992) ound that the

BPP and umbilical artery Doppler measurements are comparable indicators of fetal acidosis. Introduction of Doppler velocimetry as a primary screening test must balance any advantages

against its necessary training and associated costs.

In addition, umbilical artery Doppler velocimetry does

not efectively predict etal health in normal pregnancies

(Alrevic, 2015; Page, 2017). Gonzalez and associates (2007)

found that abnormal umbilical artery Doppler ndings in a

cohort o growth-restricted etuses were the best predictors

of perinatal outcomes. e American College of Obstetricians and Gynecologists (2021a) has concluded that umbilical artery Doppler velocimetry has not proved valuable as

a screening test, except in cases o etal-growth restriction

(Chap. 47, p. 830).

■ Ductus Venosus

Doppler ultrasound can also assess the fetal venous circulation,

and as discussed earlier, an abnormal ductus venosus Doppler

waveorm indicates cardiac dysunction. However, its routine

use in surveillance o etal-growth restriction is not recommended (Society or Maternal-Fetal Medicine, 2020).

Ductus venosus Doppler is also used in the staging of twintwin transfusion syndrome (Quintero, 1999). Abnormal Doppler indices reect myocardial dysunction and predict a poorer

outcome (Banek, 2003). Additionally, this Doppler method can

help monitor etuses with congenital heart deects and supraventricular tachycardia (SV) (Seravalli, 2016). Namely, SV can

induce a reversible cardiomyopathy that may lead to hydrops.392 The Fetal Patient

Section 6

Ductus venosus Doppler patterns may aid prediction and monitor improvement ollowing treatment. Tus, ductus venosus

Doppler may have a role in monitoring pregnancies at increased

risk or etal cardiovascular decline (Baschat, 2010).

■ Middle Cerebral Artery

Doppler velocimetry of the MCA is the primary method of

detecting fetal anemia (Chap. 14, p. 263). With fetal anemia,

the peak systolic velocity is enhanced due to greater cardiac output and decreased blood viscosity. However, to detect etal

compromise, Doppler velocimetry of the MCA is not recommended (Morris, 2012). In one randomized study o 665

women assigned to modied BPP alone or to modied BPP

plus MCA and umbilical artery Doppler velocimetry, pregnancy outcomes did not dier (Ott, 1998).

■ Uterine Artery

Vascular resistance in the uterine circulation normally declines

in the rst half of pregnancy. is stems from trophoblast

invasion and remodeling o maternal uterine vessels (Chap. 5,

p. 90). Uterine artery Doppler velocimetry may be most help-

ul in assessing pregnancies at high risk o uteroplacental insu-

ciency (Abramowicz, 2008). Persistence or development of

high-resistance patterns has been linked to various pregnancy

complications (Sciscione, 2009; Velathur, 2014). In a study of

30,519 unselected British women, uterine artery velocimetry

was assessed at 22 to 24 weeks’ gestation (Smith, 2007). e

risk o etal death beore 32 weeks, when associated with abruption, preeclampsia, or fetal-growth restriction, was signicantly

linked to high-resistance ow. However, technique standards

and criteria to dene an abnormal test are lacking. us, uterine artery Doppler studies are not considered standard practice

in either low- or high-risk populations (Society or MaternalFetal Medicine, 2020).

ANTENATAL TESTING SUMMARY

Despite a continuous evolution of options, the precision of any

given method is limited. Moreover, the wide range o normal

biological etal variation makes interpretation o test results

challenging. Tis prompts many clinicians to use antenatal testing to orecast etal wellness rather than illness.

Antenatal testing ecacy was reviewed between 1971 and

1985 at Los Angeles County Hospital (Platt, 1987). Nearly

17,000 women underwent antepartum testing o various types.

Fetal surveillance rose rom <1 percent o pregnancies in the

early 1970s to 15 percent in the mid-1980s. Tese authors

concluded that such testing was clearly benecial because the

fetal death rate was signicantly less in the tested high-risk

pregnancies compared with the rate in those not tested. Te

study, however, did not consider other innovations incorporated into practice during those years. Results from Ghana

suggest that NSTs may be benecial in low-resource countries (Lawrence, 2016). Namely, in an observational study o

316 pregnancies complicated by gestational hypertension,

women undergoing an NST had a nonsignicant decreased

risk for stillbirth compared with those not tested—3.6 versus

9.2 percent, respectively. However, the benets of antenatal fetal

testing have not been suciently evaluated in randomized trials.

Another important and unanswered question is whether antepartum fetal surveillance identies fetal asphyxia early enough to

prevent brain damage. Manning and associates (1998) studied

the incidence o cerebral palsy in 26,290 high-risk pregnancies

managed with serial BPP testing. Tese outcomes were compared with those o 58,657 low-risk pregnancies in which antepartum testing was not perormed. Te rate o cerebral palsy was

1.3 per 1000 in tested pregnancies compared with 4.7 per 1000

in untested women. odd and colleagues (1992) attempted to

correlate cognitive development in inants up to age 2 years

following abnormal umbilical artery Doppler velocimetry or

NST results. Only abnormal NST results were associated with

marginally poorer cognitive outcomes. Tese investigators concluded that by the time etal compromise is diagnosed with

antenatal testing, etal damage has already been sustained. Low

and coworkers (2003) reached a similar conclusion.

Indications or antepartum testing include etal and maternal conditions that increase the risk or stillbirth (Table 20-4)

(American College of Obstetricians and Gynecologists, 2021b).

According to the American College of Obstetricians and Gynecologists (2021a), a normal antepartum etal test result is highly

reassuring that a stillbirth will not occur within 1 week. Tis

conclusion was reached ater an analysis o reports o stillbirth

rates associated with the various antepartum etal heart rate

tests (Table 20-5).

Te most important consideration in deciding when to

begin antepartum testing is the prognosis or neonatal survival.

Te severity o maternal disease is another. In general, with

most high-risk pregnancies, testing begins by 32 to 34 weeks’

gestation. Pregnancies with severe complications, such as fetal-growth restriction, might require testing as early as 26 to

28 weeks. e frequency for repeating tests is arbitrarily set at

7 days, but more frequent testing is often done.

TABLE 20-4. Indications for Antepartum Testing

Maternal

Chronic hypertension

Pregestational DM

SLE

Antiphospholipid

syndrome

Hemoglobinopathies

Cyanotic heart disease

Cardiomyopathy

Cystic fibrosis

Restrictive lung disease

Chronic renal disease

Hyperthyroidism

In vitro fertilization

Substance abuse

Chemotherapy (current)

Prepregnancy BMI ≥35

Maternal age >35

Pregnancy-related

Gestational hypertension

Preeclampsia

Insulin-requiring gestational DM

Oligohydramnios

Polyhydramnios

Postterm pregnancy

Prior stillbirth

Isoimmunization

Cholestasis

Velamentous cord insertion

Single umbilical artery

Fetal

Fetal-growth restriction

Decreased fetal movement

Multifetal gestation

BMI = body mass index; DM = diabetes mellitus;

SLE = systemic lupus erythematosus.

TABLE 20-5. Stillbirth Rates within 1 Week of a Normal

Antepartum Fetal Surveillance Test

Antepartum Fetal Test

Stillbirtha

Rate/1000 Number

Nonstress test 1.9 5861

Contraction stress test 0.3 12,656

Biophysical profile 0.8 44,828

Modified biophysical profile 0.8 54,617

aCorrected for lethal anomalies and unpredictable causes

of fetal death such as abruption or cord accident.



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