Case 1 A 24-year-old woman with vaginal bleeding in early pregnancy, 27

Anna Smith, a 24-year-old para 0+1 is brought in to the early pregnancy assessment unit by her husband with fresh vaginal bleeding. Her last period was 6 weeks ago. She is very anxious as she had a similar episode in a previous pregnancy and miscarried.

What should you do on initial assessment?

You need to ensure that Mrs Smith is haemodynamically stable (ổn định huyết động). This initial assessment follows the basic principles of ABC. You must assess her bleeding. If she is bleeding heavily (chảy máu nhiều) and is tachycardic (nhịp chậm) or hypotensive (hạ huyết áp) then IV access should be obtained along with fluid replacement before obtaining a detailed history.

What differential diagnosis immediately comes to mind?

• Miscarriage

• Delayed period

• Ectopic pregnancy

• Molar pregnancy

• Cervical lesion

What would you like to elicit from the history?

History of presenting complaint

1 Bleeding

a. Amount and colour of bleeding

b. History of passage of any fleshy tissues/clots

c. How long she has been bleeding?

2 Pain

a. Is bleeding associated with pain?

b. Site, severity, type and radiation of pain

c. History of shoulder tip pain

3 About menstrual cycle

a. When was her last menstrual period?

b. How regular were the cycles?

c. Was she planning the pregnancy?

d. Has she carried out a pregnancy test?

4 Contraceptive history

5 Previous obstetric history

Outcome of the previous pregnancy (miscarriage, ectopic)

6 Past history

a. Previous pelvic inflammatory disease

b. Sexually transmitted infections

Mrs Smith tells you that she had noticed fresh red blood which was only spotting on wiping herself. It was associated with cramping lower abdominal pain. She describes the pain as mild in intensity, non-radiating and present for the last hour. She has not taken any analgesic as yet. A urine pregnancy test which she did at home 2 days ago was positive. She has a regular κ = 5/30–35 day cycle. Her last period was 6 weeks ago.

Her previous pregnancy was a spontaneous conception 8 months ago. Unfortunately, she had a miscarriage at 7 weeks after an episode of spotting at 6 weeks’ gestation. She is terrified that this is going to happen again. She and her partner have been trying to conceive since her last miscarriage.

What would you look for in physical examination?

General examination

Pallor, tachycardia and hypotension can be found with very heavy bleeding but this is uncommon.

Abdominal examination

After your initial assessment, you need to do an abdominal examination to detect any palpable mass. Any point of tenderness, guarding or rigidity should be elicited. Tenderness and guarding can be present in cases of ectopic pregnancy because of intraperitoneal bleeding.

Molar pregnancy can present with uterine size more than the period of gestation but is now usually diagnosed on ultrasound scan before this stage.

Pelvic examination

Look for signs of bleeding and assess how much she is bleeding. If she is wearing a pad, note if the pad is soaked or if her underwear is stained. You should note if there is active bleeding with blood trickling as this is a sign of a significant bleed.

Speculum examination

• Gently insert the speculum and see if there is bleeding in the vagina

• Use a sponge on sponge holder to see if there is any fresh bleeding

• If bleeding is seen you need to identify if it is heavy

• Check the cervical os:  look to see whether the external os is open or closed and look for any products of conception

• Check for any local lesion such as a polyp or cervical erosion

What is the clinical significance of the cervical os and the bleeding?

Inevitable miscarriage -> There is persistent bleeding. Ballooning of the cervix results from products of conception in the cervical canal and the external os is open. The process is inevitable miscarriage and this is irreversible.

Incomplete miscarriage -> There is a history of heavier bleeding with passage of clots or products of conception. The external os is open and products can be removed.

Missed miscarriage (early fetal demise) -> If bleeding is present, it is likely to be a dark brown discharge and the os is closed. This may also be the situation in ectopic pregnancy.

Now review your clinical findings.

She had her last miscarriage when she was on holiday, and no hospital records are available. At present she is haemodynamically stable. She does not look pale and her pulse is 70 beats/minute. She is up to date with her cervical smears and the last one, taken 2 months ago, was reported as normal.

Her uterus is not palpable on abdominal examination.

There is tenderness in the suprapubic area but there is no

guarding or rigidity. The cervical os is closed and there is a

small amount of fresh blood on her pad. No further

bleeding can be seen from the external os. Her cervix is long

with no evidence of ballooning. Bimanual examination does

not reveal any mass or tenderness.

What investigations would you do?

1 Full blood count. A full blood count will assess if she has had a significant blood loss.

2 Blood group and save. Because she had her last miscarriage elsewhere we do not have any records of her blood group. You need to identify if she is rhesus negative as if she has further heavy bleeding she may require transfusion.

To summarize findings so far, Mrs Smith is spontaneously

pregnant for the second time with 6 weeks’ amenorrhoea,

vaginal bleeding and lower abdomen pain. There is no

history of passage of clots or products of conception. Her

pregnancy test is positive. She is haemodynamically stable.

Pelvic examination reveals no active bleeding and a closed

cervical os. There is no abdominal tenderness, guarding or

rigidity.

What will you do next?

An ultrasound scan of her pelvis. This should be transvaginal to obtain a clear view of the uterine cavity and its

contents at her early gestation. You should warn her that

we might not be able to see a pregnancy even with a

transvaginal scan, even if it is intrauterine, because of her

early gestation. Although she gives a history of 6 weeks ’

amenorrhoea, she may be only 5 weeks ’ pregnant as her

cycle length is 30 – 35 days. Explain that transvaginal

ultrasound is not harmful to pregnancy and by itself will

not cause a miscarriage.

Pelvic ultrasound scan

A viable pregnancy can be seen from 5 weeks by transvaginal scan. A pelvic ultrasound scan of the pelvis is

helpful in ruling out ectopic pregnancy. The presence of

an intrauterine pregnancy almost always rules out ectopic

pregnancy. There is a 1 in 40,000 chance of a heterotrophic pregnancy (both an intrauterine and an extrauterine

pregnancy) in a spontaneous conception.

What ultrasound features would you

look for to diagnose a pregnancyrelated cause for her bleeding?

Ectopic pregnancy

If the uterine cavity is empty or if there is no definite sign

of intrauterine pregnancy (presence of at least a yolk sac

or fetal pole), you need to consider ectopic pregnancy. A

pseudogestational intrauterine sac is seen in 10 – 20% of

cases of ectopic pregnancy. There is a collection of fluid

inside the uterine cavity as a result of inflammatory reaction to the pregnancy hormones. There are subtle differentiating signs like contour of the sac, presence of a

double ring and eccentric position which favours the

diagnosis of a true sac rather than a pseudosac. However,

the presence of a yolk sac is a sign of true intrauterine

gestational sac and therefore intrauterine pregnancy.

Other features of ectopic pregnancy on ultrasound are a

complex adnexal cystic mass and free fluid in the peritoneal cavity (Box 1.1 ).

Ultrasound features of a viable pregnancy

In addition to the presence of an intrauterine gestation

sac with yolk sac and fetal pole, there should be presence

of a fetal heart (seen pulsating) to call it a viable

pregnancy.

Ultrasound features of molar pregnancy

The uterus is enlarged in size and reveals the classic

snowstorm appearance of mixed echogenic appearance

indicating hydropic villi and intrauterine haemorrhage.

The β human chorionic gonadotrophin (βHCG) level

can be markedly raised. Large benign theca lutein cysts

(caused by ovarian stimulation with βHCG) are seen in

20% of cases.

Her pelvic ultrasound scan reveals a thickened endometrium.

A very small 2-mm sac is seen which cannot be

differentiated from a pseudosac. There was no evidence of

fetal pole or yolk sac. Her right ovary is normal and the left

ovary shows a 2 × 2.5cm cyst. There is minimal free fluid in

the pouch of Douglas and an ectopic pregnancy cannot be

ruled out.

Can you explain her pelvic

scan findings?

You cannot detect a definite sign of intrauterine pregnancy. It may be that it is a very early pregnancy or an

Figure 1.1 Pelvic ultrasound scan of an intrauterine pregnancy.

Box 1.1 Early embryology

Implantation in the uterine cavity occurs around cycle day

21, once the blastocyst enters the uterine cavity. Once the

blastocyst comes in contact with decidualized

endometrium, the trophoblast proliferates and

differentiates into two layers: cytotrophoblast and

syncytiotrophoblast (cells in contact with endometrium).

Two layers of cytotrophoblast are separated by

extraembryonic mesoderm

Gestational sac

A gestational sac is usually imaged as a round or oval

anechoeic structure and can be seen as early as 20–23

days after ovulation. This develops from isolated spaces in

the extraembryonic mesoderm fusing and forming a simple

fluid-filled cavity that contains amnion, yolk sac and

embryo

Yolk sac

Yolk sac, a spherical structure, develops on cycle day 28,

when the extracoelomic membrane extends completely

around the inner wall of the blastocyst forming a

secondary cavity. It can be seen on transvaginal scan by 5

weeks. When present, the yolk sac confirms the diagnosis

of intrauterine pregnancy

KEY POINT

An empty uterus on scan might represent a complete

miscarriage, very early intrauterine pregnancy or an ectopic

pregnancy

ectopic pregnancy. The cyst in the left ovary is probably

a corpus luteum. Hence you need to investigate further.

What further investigation would you

do now?

Although she has a positive urinary pregnancy test, an

ectopic pregnancy cannot be ruled out on ultrasound and

a serum βHCG is advised.

Explain to her the rationale for

a serum βHCG?

See Box 1.2 .

Now review her test results

βHCG result shows a value of 412IU/L and her blood group

result shows that she is group O rhesus negative. Her full

blood count is normal.

What information do you need to give

to her?

• Despite high resolution ultrasound, you may not be

able to see evidence of intrauterine pregnancy at transvaginal ultrasound scan as the levels of βHCG are less

than 1000 IU/L

• A level of 1000 IU/L is taken as a discriminatory level

to be able to see an intrauterine gestation sac

• A repeat βHCG in 48 hours will help in this case to

determine whether it is an ongoing pregnancy or not

• A single βHCG gives very limited information unless

the levels are high

What is the clinical significance of her

being rhesus negative?

Non - sensitized rhesus - negative women need to receive

anti - D immunoglobulin in the following situations to

prevent the development of anti - rhesus antibodies:

• Ectopic pregnancy

• All miscarriages over 12 weeks ’ gestation (including

threatened)

• All miscarriages where the uterus is evacuated (whether

medically or surgically)

• Threatened miscarriage under 12 weeks ’ gestation only

when bleeding is heavy or associated with pain

• Complete miscarriage under 12 weeks ’ gestation only

when there is formal intervention to evacuate the uterus

Does Anna require anti-D

immunoglobulin?

She does not need anti - D immunoglobulin in her present

situation but once a diagnosis has been made this may

need to be reviewed. She can go home and return after

48 hours.

She returns 48 hours later for a repeat βHCG having had a

further episode of spotting in the morning. The repeat blood

test shows a value of 880IU/L.

Review all your differential diagnoses

Threatened m iscarriage. This is the most likely diagnosis

so far. There is only a small amount of bleeding, the

cervical os is closed and βHCG has doubled in 48 hours.

Inevitable m iscarriage. Incomplete miscarriage means that

the process of miscarriage has started and some products

of conception have been expelled while the rest still

remains. This is unlikely in this case as the os is closed and

bleeding has stopped (apart from one episode of spotting).

In inevitable miscarriage, women continue to experience

cramping abdominal pain as the uterus is contracting and

trying to expel the products. Moreover, the bleeding continues, gradually becoming heavier and you would see an

open os on speculum examination. Although threatened

miscarriage can progress to inevitable miscarriage, at

present in this case there are no such signs.

Box 1.2 β subunit of human chorionic

gonadotrophin

• This is a glycoprotein consisting of α and β subunits

secreted by trophoblast tissue which gives us an idea of

where the pregnancy is

• The βHCG should double in 48 hours in cases of an

ongoing intrauterine pregnancy

• It rises, but less than double, in ectopic pregnancy

(66%)

• If it falls to half in 48 hours it suggests miscarriage

• In 15% of normal intrauterine pregnancy the βHCG will

rise less than double

• In 15% of ectopic pregnancies, a doubling will be seen

in 48 hours

• Failure of βHCG to double in absence of an intrauterine

pregnancy suggests the diagnosis of ectopic pregnancy

• In cases of molar pregnancy, very high levels of βHCG

can be seen

• When considering the test results, the whole clinical

situation must be taken into consideration

Incomplete m iscarriage. This is unlikely as Mrs Smith

does not give a history of passing clots or products of

conception. Moreover, the βHCG has doubled, indicating it is an ongoing pregnancy. In the case of miscarriage

it should decrease, ideally to half in 48 hours. There is a

risk of infection if products are left.

Complete m iscarriage. This could be a possibility as the cervical os is closed and bleeding has stopped. However, there

is no history of passage of clots or products of conception.

Missed m iscarriage. This was a possibility initially but it

was ruled out with the doubling βHCG. As the pregnancy

stops growing, the βHCG levels will reduce. The symptoms of pregnancy may disappear.

Recurrent m iscarriage. This is defined as the loss of three

or more pregnancies consecutively. It affects 1% of women.

In this case there is only one previous miscarriage, hence

investigations for recurrent miscarriage (karyotype of both

partners; lupus anticoagulant; anticardiolipin antibodies;

thrombophilia screen) are not needed.

Ectopic p regnancy. Although initially plausible, this is

unlikely as βHCG is doubled in 48 hours and there are no

risk factors for ectopic pregnancy (previous ectopic, history

of pelvic inflammatory diseases). Moreover, Anna is haemodynamically stable and there is no abdominal guarding

and rigidity. However, it cannot be excluded until an intrauterine pregnancy is demonstrated on ultrasound.

Molar p regnancy. βHCG is too low for it to be a molar

pregnancy, her uterus is not enlarged inappropriately to

the period of gestation and there is no history of passing

vesicles.

Bleeding from a l ocal l esion. This has been excluded as

there no evidence of cervical ectopy or polyp on

examination.

What would you tell this patient?

• You should reassure her because her serum βHCG has

doubled in 48 hours

• It is most likely an early ongoing intrauterine pregnancy as she may be only 5 weeks ’ pregnant considering

her 30 – 35 day cycle

• Ultrasound scan is not useful at present as the values

of βHCG are still less than 1000 IU/L

• She should be advised to come back in a week ’ s time

for an ultrasound scan

• A 24 - hour contact number for the early pregnancy

assessment unit should be given in case she has further

questions

• Further support should be offered as the couple are

very anxious

• You need to explain to her that one miscarriage is very

common. Up to 15 – 20% of pregnancies miscarry

• Investigations for miscarriage are not advised until

a couple has had at least three consecutive

miscarriages

• One miscarriage does not alter the outcome in future

pregnancies

Anna returns in a week and her pelvic ultrasound now

shows a single intrauterine gestation sac with yolk sac and

fetal pole with a heart beat present.

What is the outlook now?

Her scan has confirmed a continuing intrauterine pregnancy. She will need more reassurance at least until her

scan after 8 weeks, which is beyond the gestation of

her last miscarriage. She should have a 24 - hour contact

number in case she bleeds heavily. Approximately

15% of pregnancies are complicated by a threatened

miscarriage.

CASE REVIEW
This case of a very anxious couple is typical of women who
present with very light bleeding in very early pregnancy.
Her reason for anxiety is primarily because of her previous
history of miscarriage. At every stage in history - taking and
examination it is important to remember that she wishes
something to be done to prevent miscarriage happening
again.
Despite her history it is important to rule out other
causes of early pregnancy bleeding. History, examination
and investigations in this case revealed a likely diagnosis of
threatened miscarriage, although ectopic pregnancy could
not be ruled out until the intrauterine pregnancy was demonstrated. Although transvaginal ultrasound can demonstrate intrauterine pregnancy from 5½ weeks onwards,
sometimes dates are mistaken and hence the actual period
of gestation when prior menstrual cycles have been longer
(30 – 35 days in this case).
There is no reason to keep her in hospital and an
outpatient management is justified. Admission will not
prevent the miscarriage if it were to occur again. She has
been given a 24 - hour contact number in case of heavy
bleeding or pain


KEY POINTS

• ABC of resuscitation forms the initial assessment for

bleeding in pregnancy

• Ectopic pregnancy needs to be excluded in women with

pain or bleeding in early pregnancy

• Ectopic pregnancy and miscarriage can present without

the patient even realizing that she is pregnant

• Understand that psychological support is equally

important as the medical management of the condition

• Follow-up for counselling may be required, especially as

this is a repeat episode

• Support groups, a point of contact and follow-up

reassurance scans will be needed

• Investigations for recurrent miscarriages are not warranted

unless there are three consecutive miscarriages

• Even after two miscarriages there is 80% chance of

having a live baby

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